CLIQS Frequently asked questions
If you can't find your answer below, contact us directly.
Can I use the software on multiple computers
Can I install on a machine with no internet connection?
How can I purchase the software?
I have further questions. Can I have a demonstration?
Is it possible to have a trial of CLIQS?
I haven't had a chance to use the trial can I have an extension?
What Operating Systems can I use?
Why should I pay money for software?
Why does Automatic Lane Creation fail on my gel?
There could be a number of reasons this happens. It could be because
- The lanes are short or have very few bands
- The lanes are too close together so the algorithm cannot split them
- The lanes are not quite the same width which is an assumption the algorithm uses
- The resolution of the image is very low and there is not enough band information
Using an AOI for lane detection can help (just draw a box when in automatic detection mode) or simply try to create the lanes manually.
How does Molecular Sizing work?
Why use different curve types?
The default curve fit is cubic spline. This curve type creates a smooth curve passing through all points. It has no defining equation. This option can be somewhat over sensitive to inaccurate mappings but will guarantee that the results are as expected from where the iso-molecular weight lines(yellow) cross the lanes. First order Lagrange is similar but uses straight lines.
The other main option used historically is the Linear Log equation which is a straight line calculated from the log of the Molecular Size. This is theoretically correct but can lead to misleading results due to any inaccuracy of the curve fit. Even curves with high R2 results can still have problems.
What is the name of the curve fitting algorithym?
My image does not look like the image I captured. Why?
A histogram of all pixel values is created. Then the default auto contrast is created from the value of the histogram after 0.5% of all pixels and at 99.5% of all pixels. This stops outliers or scanner noise affecting the result but it can look a little different
If the image has a calibration (from Powerscan) or an encoding (from GE or Fuji scanners) then the converted values are used by default for the image. If you do not want this then use the “Raw” option to go back to the raw pixels values
Note: Contrast does not affect the values used in any calculations
My profile is upside down. What can I do to fix it?
What file formats can the software support?
When I load in my JPG file the image is inverted.
I cannot load my tiff image?
What is Band Volume?
Where can you get the total volume of all the bands in a Lane?
What is the background level in Array and why don't all spots have the same Background Volume?
What is Profile Deconvolution?
How do I turn Profile Deconvolution on?
By default in CLIQS this option is off. You can turn the option on from the start or finish pages of the wizard by ticking the check box next to Profile Deconvolution.