CLIQS – Technical Specification

CLIQS – Core Laboratory Image Quantification Software

Four essential image analysis tools for every lab

  • 1D electrophoresis gels and Western Blots
  • High-density DNA and protein arrays
  • Colony counting & basic 2D spot measurement
  • General feature-based image analysis

How CLIQS software works

For 1D gels, highly developed algorithms accurately detect lanes and bands even on distorted gel images. Results can be verified using the range of visualisation tools which aid further examination of lane and band data.
Calibrate the bands using one or more Molecular Size standard lanes and derive accurate quantitation from known band volumes
For the Arrays and Colony plates, rapid identification and quantitative measurements of all relevant features allow for speedy analysis and accurate data
All common image formats can be analysed, maximising the image capture instruments you already have in your lab.

Why CLIQS?

Easy to use

Our tools are designed with you in mind. Each program mode clearly shows all the options available and editing is straight forward when you need it.

Fast, automated analysis

Speed up your work and make your results less subjective with automated detection of lanes, bands, spots and array grids. Automatic background subtraction options eliminate image noise from measurements.

Data Export and Reports

Use the Clip Gallery tool to save publication quality images (300dpi) at each step of your analysis. All tables, images, results are exportable in a variety of formats.

World Class Support

All products come with a year’s free support. We aim to reply to all support queries within 24hrs and will fast turnaround for updates and fixes.

Continual Development

The products are developed on a regular basis to cover operating system changes, new techniques and improving your experience.

GLP version

A special version of CLIQS is available to help labs meet their GLP requirements for FDA 21 CFR Part 11.

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Analysis Features

Image pre-processing

  • Invert colours
  • Flip, crop and rotate
  • Optimise contrast and brightness

1D Gel Automation

  • Automatic analysis with Protocols – saved parameters for repeat analysis

Basic 1D gel quantification

  • Automatic and manual lane creation
  • Add grimaces to account for band distortion
  • Multi-tier analysis
  • 8 methods of Background subtraction to remove noise tailored to your gels
  • Automatic Band Detection with easy to use editing
  • Molecular Size calibration

Advanced 1D gel quantification

  • Profile Deconvolution : Fit Gaussian curves to profile
  • 4 methods of Quantity Calibration to assign known quantified values to bands
  • Normalise the band volumes

1D multiplex quantification

  • Create and load multiplex gels from up to 4 channel images
  • Propagate Molecular Size results across channels
  • Normalise using Housekeeping proteins/genes
  • Normalise using Total Protein Normalisation

1D Lane comparison

  • Calibrate Rf of lanes to adjust for gel smiling
  • Single gel band matching to compare DNA lanes
  • Band Matrix and Lane similarity results table
  • UPGMA and Neighbour Joining Dendrogram creation

21 CFR Part11 version

  • 1D gel analysis results stored in secure folder
  • Windows security for usernames and passwords
  • Audit Trails of all work
  • Electronic Signatures for signing off work

Array Analysis

  • Automatic and manual grid detection up to 1536 spots
  • Flexible and easy to use grid and spot editing
  • 4 methods of Background subtraction to remove noise tailored to your images
  • Normalisation of spot volumes
  • Presence/Absence Flagging based on volume threshold

Colony Counting

  • Automatic colony detection in circular or rectangular AOI
  • Easy to use Editing tools to draw, delete or split features
  • 2 methods of Background subtraction to remove noise

General Image Analysis

  • Draw and measure rectangles, polygons, ellipses and grids on any image
  • 4 methods of Background subtraction to remove noise tailored to your images
  • Flexible editing options including grouping of measurements

Exploring Results

  • Wide range of data fields to display in measurements tables
  • Export and Print all image and table results
  • Annotate images
  • 3D view of image
  • Clip Gallery to capture publication quality images
  • PDF Reports for 1D gel analysis

Multiplex analysis of Western blots using loading controls for accurate quantification

  • CLIQS is hardware-independent so you can analyse all common image files used for multiplexing.
  • Once multiplex images are opened or created follow the straightforward lane, background and band detection steps.
  • If you have housekeeping proteins then make sure they are detected on the normalisation channel. Then just choose the loading control normalisation method you need and the results are calculated automatically.
  • You can then view and export publication quality images and export the normalised data as required.
1D gel image: House-keeping protein GAPDH (green) is measured in one channel and used to normalise a dilution series for a protein of interest in a second channel with a MW marker in the first lane (red)

CLIQS FAQs

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