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CLIQS – Technical Specification

CLIQS – Core Laboratory Image Quantification Software

Four essential image analysis tools for every lab

  • 1D electrophoresis gels and Western Blots
  • High-density DNA and protein arrays
  • Colony counting & basic 2D spot measurement
  • General feature-based image analysis


PC Specifications

Operating systems: Windows Vista, Windows 7, 8 and 10.
Processor: 1.4 GHz
Memory: Minimum 1Gb, recommended 2Gb
Free hard disk space: Recommended 5Gb
File formats: .tif,.gel,.jpg,.bmp,.gif,.png,.img (Fuji)

Core Laboratory Image Quantitation Software

How CLIQS software works

For 1D gels, highly developed algorithms accurately detect lanes and bands even on distorted gel images. Results can be verified using the range of visualisation tools which aid further examination of lane and band data.

Algorithms accurately detect lanes and bands.

Calibrate the bands using one or more Molecular Size standard lanes and derive accurate quantitation from known band volumes

Molecular weight and band volume calibration is available within the software

For the Arrays and Colony plates, rapid identification and quantitative measurements of all relevant features allow for speedy analysis and accurate data

Array analysis and colony counter also available within the software.

All common image formats can be analysed, maximising the image capture instruments you already have in your lab.

CLIQS is vendor independent, analysing all common image formats.


Easy to use

Our tools are designed with you in mind. Each program mode clearly shows all the options available and editing is straight forward when you need it.

The software is easy to use with a comprehensive manual included.

Fast, automated analysis

Speed up your work and make your results less subjective with automated detection of lanes, bands, spots and array grids. Automatic background subtraction options eliminate image noise from measurements.

Automatic lane and band detection driven by advanced algorithms.

Data Export and Reports

Use the Clip Gallery tool to save publication quality images (300dpi) at each step of your analysis. All tables, images, results are exportable in a variety of formats.

Easy data export and high resolution, poster ready, images available.

World Class Support

All products come with a year’s free support. We aim to reply to all support queries within 24hrs and will fast turnaround for updates and fixes.

Get one year free support with a purchase of CLIQS

GLP version

A special version of CLIQS is available to help labs meet their GLP requirements for FDA 21 CFR Part 11.
Find out more about CLIQS 1D 21 CFR here.

CLIQS 1D 21 CFR version for your GLP standard labs.



Analysis Features

Image pre-processing

  • Invert colours
  • Flip, crop and rotate
  • Optimise contrast and brightness
Image pre-processing available

1D Gel Automation

  • Automatic analysis with Protocols – saved parameters for repeat analysis
Create and run an automated analysis protocol.

Basic 1D gel quantification

  • Automatic and manual lane creation
  • Add grimaces to account for band distortion
  • Multi-tier analysis
  • 8 methods of Background subtraction to remove noise tailored to your gels
  • Automatic Band Detection with easy to use editing
  • Molecular Size calibration
Automatic lane and band detection.

Advanced 1D gel quantification

  • Profile Deconvolution : Fit Gaussian curves to profile
  • 4 methods of Quantity Calibration to assign known quantified values to bands
  • Normalise the band volumes
Band quantification with 4 methods of quantity calibration available.

1D multiplex quantification

  • Create and load multiplex gels from up to 4 channel images
  • Propagate Molecular Size results across channels
  • Normalise using Housekeeping proteins/genes
  • Normalise using Total Protein Normalisation
Up to four multiplex channels available in a single image.

1D Lane comparison

  • Calibrate Rf of lanes to adjust for gel smiling
  • Single gel band matching to compare DNA lanes
  • Band Matrix and Lane similarity results table
  • UPGMA and Neighbour Joining Dendrogram creation
Single gel lane comparisons available in CLIQS

21 CFR Part11 version

  • 1D gel analysis results stored in secure folder
  • Windows security for usernames and passwords
  • Audit Trails of all work
  • Electronic Signatures for signing off work
Good Lab Practice version available for compliance with FDA regulations

Array Analysis

  • Automatic and manual grid detection up to 1536 spots
  • Flexible and easy to use grid and spot editing
  • 4 methods of Background subtraction to remove noise tailored to your images
  • Normalisation of spot volumes
  • Presence/Absence Flagging based on volume threshold
Automatic analysis of up to 1536 spots.

Colony Counting

  • Automatic colony detection in circular or rectangular AOI
  • Easy to use Editing tools to draw, delete or split features
  • 2 methods of Background subtraction to remove noise
Automatic colony detection and counting.

General Image Analysis

  • Draw and measure rectangles, polygons, ellipses and grids on any image
  • 4 methods of Background subtraction to remove noise tailored to your images
  • Flexible editing options including grouping of measurements
Essential tools for easy image analysis of any images.

Exploring Results

  • Wide range of data fields to display in measurements tables
  • Export and Print all image and table results
  • Annotate images
  • 3D view of image
  • Clip Gallery to capture publication quality images
  • PDF Reports for 1D gel analysis
PDF reports, image export and more for the presentation of data.

Multiplex analysis of Western blots using loading controls for accurate quantification

  • CLIQS is hardware-independent so you can analyse all common image files used for multiplexing.
  • Once multiplex images are opened or created follow the straightforward lane, background and band detection steps.
  • If you have housekeeping proteins then make sure they are detected on the normalisation channel. Then just choose the loading control normalisation method you need and the results are calculated automatically.
  • You can then view and export publication quality images and export the normalised data as required.
Multiplex analysis of Western blots available through CLIQS

1D gel image: House-keeping protein GAPDH (green) is measured in one channel and used to normalise a dilution series for a protein of interest in a second channel with a MW marker in the first lane (red)