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The image saturation effect on image analysis

What is saturation?

Saturation occurs when grey levels exceed the maximum available. When a gel image becomes too saturated, any differences in high pixel intensities cannot be resolved, and the saturated spots appears truncated when viewed in 3D.

Why can saturation be a problem?

The spot detection is not optimised for the unnatural shape of these spots, since the peaks are effectively ‘missing’ in the scanned image no reliable quantitation can be done with them.

The video below illustrates how saturation can look in the image analysis software and what you can do to avoid saturation.

 

We’ve created some more useful documents discussing quantitation

How can I capture my images so my analysis is quantitative?

Capturing high dynamic range with gel doc systems

Capturing bit depth with gel doc systems