2D gel electrophoresis followed by Western blotting for host cell protein coverage is a powerful technique that can deliver additional discovery into the capability of an anti-HCP antibody. However there are still additional challenges in your 2D Western blot analysis experiment. The quality of spot detection greatly effects the percentage coverage results you obtain from your experiment, spots with varying intensities must be accurately quantified in order to obtain meaningful results.
In this video we show you how automatic spot outlines in SpotMap obtain an objective, accurate and quantitative analysis and how you can use 3D mode to validate the software you currently use for HCP coverage.