A dot blot array, quantified in Phoretix Array

Phoretix Array

1 / 6 · Open

Array images from any imager.

Single or multichannel images, one channel per dye or stain, as TIFF, JPEG, PNG, BMP or IMG files.

Phoretix Array

Dot blot, array and plate analysis. Fit the grid once, save it as a template, and the next image goes straight to results, in under a minute.

Start a free trial at totallab.com

Your software won’t do what you need? Ask TotalLab.

1. Open any array image

   Dot blots, cytokine arrays, ELISpot and FluoroSpot plates, slot blots, ELISA and ELIFA plates, In-Cell Westerns, microtiter plates and DNA or RNA microarrays, from fluorescence, chemiluminescence or colorimetric detection. Multichannel images are supported, with one channel for each stain, color or dye, for multiplex blots and two-color microarrays. TIFF, IMG, JPEG, PNG and BMP files from almost any scanner or imager.

2. Fit the grid

   Click and drag a measurement grid over the image. 96 and 384 well plate templates are built in, and measurements are taken directly from the raw image, with no compression and no scaling.

3. Background subtraction

   Choose from a range of background subtraction methods for accurate results from even the noisiest images, with calculations based on peer-reviewed methods.

4. Normalize, calibrate, call presence

   Normalize to a specific well or to the average of several, calibrate unknowns against known standards, and set a presence threshold to mark positive and negative results across the whole array.

5. Template once, run anywhere

   Your settings are saved as a template, so the next image goes straight to results. Build an unlimited library and email templates to colleagues or other sites, so every site and CDMO runs exactly the same analysis with less inter-operator variability.

6. Export and comply

   Export a PDF report or a raw CSV file for further calculation. With AuditSafe, Phoretix Array meets 21 CFR Part 11, EU Annex 11 and GMP requirements from captured image to final report, and IQ/OQ validation packages are available. Runs on Windows, macOS and Linux.

“I really liked the software, it was easy to use and gave me everything I needed for quantification of dot blots. It was the first time I did this kind of experiment and this software saved me a lot of time.”

Johnny Cabrera, Novartis