Host cell protein analysis in SpotMap MS
A 58-second loop of LC-MS host cell protein analysis, from runs taken straight from a Thermo Fisher LC-MS or a watched folder to a verified HCP report that comes out the same for every operator and site: visual QC, a deliberately sensitive search, Hi3 quantitation, high-risk flagging and peptide-level verification that clears false positives.
Automated LC-MS host cell protein analysis that casts a wide net, so no dangerous HCP slips through. DIA or DDA data in, a verified HCP report out.
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1 / 7 · Load
Data in from your instrument or a watched folder.
Stream runs straight from your Thermo Fisher LC-MS or drop them in a watched folder; add your FASTA and analysis starts on its own.
Load, hands-free
Take runs directly from your Thermo Fisher LC-MS, or assign a watched folder so new runs are analyzed automatically with your pre-set parameters, restarting where they left off if the computer stops. Add a FASTA file; DIA and DDA acquisitions are both supported.
Visual QC
Upfront data visualization shows whether a dataset is worth analyzing, so a failed technical replicate is caught while there is still time to prepare a new sample.
Identify, with a wide net
SpotMap MS searches your FASTA with deliberately sensitive settings and errs on the side of false positives rather than false negatives, because some HCPs, such as enzymes, are dangerous in vanishingly small amounts. Use your own spectral library, a commercial or public one, or TotalLab's AI-powered HCP spectral library.
Quantify
Each HCP is reported in relative units by label-free Hi3 quantitation against spiked-in standard proteins, the relative-to-spiked-in-protein approach described in USP <1132.1>.
Flag high-risk HCPs
Detected HCPs are checked against the built-in threat-level database, free with every license, or the BioPhorum HCP Data Platform. Nothing is hidden; anything suspected of being present and risky is highlighted.
Verify
The Protein Verification Loop guides peptide-level review of XIC, MS and MS/MS data, so false positives from the wide net are cleared quickly, and records which peptides were reviewed, by whom and when.
Consistent across operators and sites
Manual, parameter-heavy analysis makes it too easy for different users to get different answers. SpotMap MS automates the analysis end to end with pre-set parameters, so operators and sites running the same method get the same HCP results.
Report
A complete HCP report, produced automatically. Pair with AuditSafe for 21 CFR Part 11, EU Annex 11 and GMP, and track HCP content over time in TrendLab.
Try SpotMap MS free with your own LC-MS data. Working in a regulated lab? Pair it with AuditSafe for 21 CFR Part 11, and ask about in-person IQ/OQ validation packages.
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