How to Analyse Host Cell Protein (HCP) Antibody Coverage % Using SpotMap 2D Image Analysis Software

This tutorial will show you how to analyse 2D gel electrophoresis and western blot images using TotalLab’s SpotMap HCP image analysis software and calculate a percentage coverage score.

SpotMap is an industry-leading and complete solution for analysing HCP coverage using 2D gel electrophoresis and Western blot images.

0:00 – Intro

0:30 – How to Import Your Images

1:00 – Main Window Overview

1:40 – Contrast/Brightness Correction and False-Colour Overlays

2:50 – Image Editing Tools Within SpotMap

4:50 – Quality Control of Images

6:05 – MW Strip Calibration

06:55 – pI Strip Calibration

7:26 – Annotation of Spots

9:03 – How to Align Your Images

11:23 – Manual Alignment

14:58 – One-click Automatic Alignment

16:22 – Automatic Spot Detection

17:44 – Manual Spot Editing Tools

21:03 – Automatically Detecting New Spots

21:18 – Using Spot Sets

21:28 – Marking Spots as Present

21:52 – Marking Spots as Absent

23:01 – Filtering by Spot Intensity

24:14 – Calculating Coverage Percentage

25:05 – Automatic Spot Sets

25:57 – Defining Product Area

27:05 – Finding Significant Differences Between Spots

27:15 – Spot Measurements

28:05 – 3D View of Spots

29:00 – Exporting PDF Reports

31:00 – Example Report

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