TotalLab · CLIQS 1D Pro

Band and lane matching across hundreds of 1D gels, in CLIQS 1D Pro

A 45-second loop of CLIQS 1D Pro at scale: SDS-PAGE and immunoblot images from many runs, days and sites, lanes and bands detected on every gel, Rf calibration that corrects gel-to-gel running differences so bands match between gels, every lane stored in one database, any lane compared with any other and clustered into a dendrogram, and unknown samples identified against your reference library.

CLIQS 1D Pro

1 / 6 · Gels

Every gel you run, in one place.

SDS-PAGE and immunoblot images from different runs, days and sites.

CLIQS 1D Pro

Band and lane matching across hundreds of 1D gels, with one lane database and a reference library for identifying unknowns. For SDS-PAGE, immunoblot and DNA fingerprint images.

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  1. Every gel, in one place

    Bring in SDS-PAGE gels and immunoblots from every run, day, instrument and site, as TIFF images.

  2. Lanes and bands, automatically

    CLIQS 1D Pro detects the lanes and bands on every gel, and sizes every band from the molecular weight ladder. Any lane or band can be adjusted by hand.

  3. Matched between gels

    No two gels run exactly alike. Rf calibration against the ladder corrects gel-to-gel running differences, so bands are matched between gels, not just between lanes on one gel, within a tolerance you set.

  4. One lane database

    Every lane from every gel goes into one database, organized in separate lane sets. There is no fixed limit on lanes or gels, only your computer's memory and disk, and colleagues can open databases that other users have created.

  5. Any lane against any lane

    Compare any lane in the database with any other, whichever gel it came from. Band similarity by Dice (band sharing index) or Jaccard, shown as UPGMA dendrograms and similarity tables.

  6. Identify unknowns

    Match each new lane against a reference library to find its closest known sample, for batch consistency, protein fingerprinting or strain typing. Export PDF reports, CSV tables and dendrogram images, with 21 CFR Part 11 controls through AuditSafe.

See CLIQS 1D Pro on your own gels and blots, for batch-to-batch comparison, protein fingerprinting, strain typing and food authentication. Working under GMP? AuditSafe adds 21 CFR Part 11 and Annex 11 controls.

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