CLIQS 1D Pro | How to create dendrograms for similarity

Watch as we demonstrate how to visualise the similarities between lanes, taken from different gels or blots, in the form of dendrogams or similarity tables in CLIQS 1D Pro.

00:00 – Intro

00:16 – Selecting Lane Set to Convert to Dendrogram

00:33 – Band Matching Before Dendrogram Creation

00:48 – Clustering Screen Description

01:07 – Blank Clustering Screen

01:17 – Algorithms for Data Generation

01:23 – Create Your Dendrogram

01:38 – Create Your Similarity Tables

02:28 – Explanation of Cophenetic Correlation and Similarity Scores

CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots which can then be searched and compared to unknown samples to quickly identify them.

The algorithms embedded within CLIQS 1D Pro effectively compensate for any distortions between different gel runs to allow accurate comparisons and matching between samples as though they were all run on the same gel, at the same time.

CLIQS 1D Pro was specifically designed to facilitate research techniques such as Inter simple sequence repeat (ISSR)-PCR, PFGE bacterial DNA fingerprinting (PFGE), denaturing gradient gel electrophoresis (DDGE), restriction fragment length polymorphism, enterobacterial repetitive Intergenic Consensus (ERIC) PCR and single-strand conformation polymorphism (SSCP) electrophoresis.

For a free trial of CLIQS 1D Pro and see how well it works creating a database from your own images, please fill in the form here.

Find out more about our other image analysis-based products here.