Image analysis resources library

TotalLab – World Precision Instruments Partnership

How to Implement 21 CFR Part 11 In Your Lab

Learn how TotalLab's software implements secure electronic records, robust audit trails, and user authentication protocols. Achieve compliance seamlessly with our expert guidance

Phoretix 1D | Background Removal Techniques For Western Blot

In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.

2D DIGE vs ELISA vs AAE for HCP Analysis

Explore the current landscape of anti-HCP antibody coverage with our article comparing 2D DIGE, ELISA, and AAE. Optimise your antibody selection process for robust anti-HCP analysis in biopharmaceutical research.

2D DIGE vs LC-MS/MS

Dive into the world of precision and versatility as we explore the distinct advantages offered by 2D DIGE and LC-MS/MS for comprehensive proteomic profiling.

2D DIGE vs 2D Gel Electrophoresis

In this short guide, we'll be explaining to you the technical process of performing 2D DIGE experiments and some of the advantages of using the technique over standard 2D electrophoresis.

CLIQS 1D Pro | How to create dendrograms for similarity

CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots. Here we demonstrate how to visualise the similarities between lanes in the form of dendrogams or similarity tables.

SameSpots | How to assign protein isoelectric point and molecular weight to spots

Learn how to add pI and MW info into SameSpots for automatic calculation of molecular weight for all identified spots.

CLIQs 1D Pro | How to Accurately Match Bands/Compare Lanes Across Different Gels and Blots

Watch how CLIQS 1D Pro can perform a simple comparison between lanes taken from different gels or blots, to identify any matches between them.

SpotMap | How to Analyse Host Cell Protein (HCP) Antibody Coverage %

This tutorial will show you how to analyse 2D gel electrophoresis and western blot images using TotalLab's SpotMap HCP image analysis software and calculate a percentage coverage score.

Phoretix 1D | How to Choose the Correct Background Removal Method

In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.

Phoretix 1D | How to Automate Your Western Blot or Gel Electrophoresis Analysis

The automatic analysis features built into Phoretix 1D can save you huge amounts of time in a high-throughput environment and minimise inter-operator and inter-lab variability.

Phoretix 1D | How to Analyse Thin Layer Chromatography (TLC) Experiments

This tutorial will show you how to analyze thin layer chromatography slides/images using TotalLab's Phoretix 1D image analysis software.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Match Bands Across Your Database

In this video we show you how to perform a search of your blot or gel database to find matching bands across lanes, how to fine-tune your search parameters and how to express your matches statistically.

Phoretix 1D | How to Analyse a Western Blot

Following the launch of our Phoretix 1D image analysis software we've put together this tutorial video to show you how to analyse gel electrophoresis and blot images.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Save Sets of Lanes for Matching

In this video we'll be showing you how to create a set or group of existing lanes that you can repeatedly match across your entire 1D gel or Western blot database.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Create Lanes for Band Matching

In this video, we'll be showing you how to select an existing lane or create a new lane containing the bands you're interested in matching across your entire 1D gel or Western blot database.

CLIQS 1D Image Analysis Software | Tutorial Series | How to Analyse Lateral Flow Tests

In this video, we'll show you how you can obtain quantifiable data from lateral flow tests using CLIQS, which can be useful when designing new lateral flow tests.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Create a Database of Gel/Blot Images

In this video, we'll guide you through the first step in using our CLIQS 1D Pro software - the creation of a database containing lanes and bands extracted from 1D gels and blots analysed using our CLIQS 1D software.

SameSpots 2D Image Analysis Software | Tutorial Series | Image Quality Control

This video will show you the tools that are built directly into SameSpots to critically evaluate the quality of your 2D images.

SameSpots 2D Image Analysis Software | Tutorial Series | Multivariate Statistical Analysis of Spots

This video guides users through the statistical analysis of spots within 2D gels and Western blots using our SameSpots software.

SameSpots 2D Image Analysis Software | Tutorial Series | How to Align Images (Automatic & Manual)

This video outlines how SameSpots's next-generation image alignment algorithm is utilised to perfectly align 2D gel and Western blot images for statistical analysis in proteomic work.

Problem solving with SpotMap

Our guide covers some of the problems you can overcome when using our SpotMap software. It provides an introduction to key features, image QC, image analysis and details on the analysis element of the software.

A guide to using SameSpots

Discover the key steps of the SameSpots workflow and how it allows you to analyse 2D gels with any type of staining.

P-values, False Discovery Rate (FDR) and q-values

In this article we explore p-values, q-values and false discovery rates through example experiment results.

CLIQS factsheet

We have created this CLIQS factsheet as a summary of how the software supports 1D gel and Western blot analysis and some of its key benefits.

How to configure system security in Windows for GxP compliance

If you're operating in a 21 CFR part 11 compliance laboratory then setting up Windows security becomes a major part in setting up your system. Take a look at our tips.

Tips for setting up your electronic signature for the FDA

We have created these guidelines to support you in setting up your electronic signatures for FDA CFR part 11 compliance.

21 CFR part 11 compliance guide

We have created this 21 CFR part 11 compliance guide to outline how our GxP Module software and the customer organisation share responsibilities for achieving compliance.

How to Configure Electronic Signatures for 21 CFR/GMP Compliance

This guide provides on how you can use AuditSafe can ensure your electronic signatures are generated in compliance with 21 CFR Part 11/GMP regulations.

SpotMap | How to align Western blots to 2D gel images

Understanding when alignment is correctly implemented is easy in SpotMap as there are visual identifiers in the software that indicate when images are ready for analysis.

Correctly aligning Western blots to 2D gel images

Alignment can be changed and corrected at any point on the analysis however there are four ways to check you have Align your images correctly.

21 CFR part 11/GMP compliance checklist

We have created this 21 CFR part 11 compliance checklist to ensure you are have the right checks in place to comply with the regulations.