Over the past twenty years TotalLab has been at the forefront of 1D gel electrophoresis and Western Blot analysis software producing such legacy products as TotalLab Quant, GelQuant, TL100, TL120, TotalLab 1D and CLIQS.
Phoretix 1D is the latest evolution of our 1D Gel and Blot analysis software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time.
We have distilled our decades of experience supporting the life science industry in 1D analysis into Phoretix 1D to deliver our most advanced set of analysis tools yet, capable of analyzing images derived from any Gel Documentation, densitometer or other imaging devices in record time.
Phoretix 1D is the complete solution for analyzing gel electrophoresis and immunoblot images, it offers:
Phoretix 1D is compatible with the latest versions of both Windows (currently Windows 11) and macOS operating systems (up to and including Ventura and M1/M2/M3 processors).
Our Phoretix 1D software is is trusted by the world’s leading educational, industrial and pharmaceutical companies:
"After having used many other software for gel image analysis, including Bio-Rad's Image Lab and ImageJ, I started using Phoretix 1D out of necessity in a new role. I found it to be much more intuitive and streamlined than what I’ve used in the past. Background subtraction, band picking, and band analysis are all automated with optional manual adjustment if desired"
Garry Smith, Proliant Health and Biologicals
Phoretix 1D is the perfect choice for the quantitative, automated analysis of:
It also allows you to overcome the limitations present in other 1D analysis software, supporting extremely high-definition images over 200mb in size and multiplexed images to make sure the quality of your experiments isn’t limited by your choice of analysis software.
"Phoretix 1D allows for the fast and accurate analysis of immunoblots, supported by optimal control over quantification parameters allowing for the generation of highly reproducible data. All features are embedded within a user-friendly interface, I especially liked the background analysis features which assists in an unbiased analysis by correcting for irregular background signals."
Jacqueline Kaiser, The University of Melbourne
Phoretix 1D offers users incredibly customizable 1-click automatic analysis of 1D SDS-PAGE and Western Blot images. Whilst you’re working on your analysis, Phoretix 1D intelligently captures your analysis settings and allows you to save them as a template file to be used again on your next experiment. When it’s time to analyze your next gel or blot simply load the previous template and go straight to your results with no need for manual intervention, saving users a huge amount of manual labor and, importantly, time.
A user can build up an unlimited library of templates to cover all their routine analyses and then share them easily sent with colleagues or even other sites anywhere in the world via something as easy as an email attachment to ensure they’re all running exactly the same analysis settings. This removes the potential for operator error entirely, greatly reduces inter-operator variability therefore reducing risk, harmonizing multiple global production or research sites and for tech transfer to a CDMO/CMO.
Background noise can come from various sources, such as non-specific binding of antibodies or stains, uneven illumination, dust, scratches, or instrumentation artifacts. If not corrected, background noise can affect the intensity and shape of the bands or spots, leading to inaccurate measurements of their size, quantity, and relative expression. Therefore, it is important to perform background removal to subtract the unwanted signal from the image, before quantifying bands or spots to make sure your data is as accurate as possible.
Our Phoretix 1D software features multiple methods of background removal for our users to choose from and refine their analysis:
| Adaptive | User-Defined |
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We also support the ability to choose different background removal techniques and settings on a per-lane basis (invaluable for accurate analysis of lanes with varying background levels between them) and different background removal techniques and settings on a per-channel basis if using multichannel images. This allows the user to compensate for different background levels between different antibodies or stains used on the same gel or blot and allows for accurate quantification and total protein normalisation functions.
Phoretix 1D also features innovative noise-reduction techniques to smooth out the intensity signal of your bands or spots, resulting in cleaner data for analysis.
You can find out more about our background removal options here or watch the video below.
Didn’t capture your gel or blot image perfectly? Not a problem.
Whilst the automatic lane and band detection algorithms built into Phoretix 1D are optimized to allow users to obtain high-quality data from less than high-quality images (meaning less repeated experiments) we appreciate that some users like the ability to edit an image before analysis to rotate it or crop out any areas of stage around the gel or blot.
Phoretix 1D supports all these functions and also includes the ability to flip gels or blots vertically or horizontally. Editing the image within Phoretix 1D means that the original .TIFF tags containing image metadata are preserved which is not the case in other image editing software, this means you can keep information relating to scale, bit depth, DPI etc. intact.
Our software is device agnostic, so can reduce costs as it can be used with all of your existing laboratory equipment regardless of the manufacturer or any additional equipment gained during future business acquisitions.
Phoretix 1D is compatible with (but not limited to) gel documentation equipment produced by Bio-Rad, Cytiva, Azure Biosystems, Thermo Fisher Scientific, Syngene, Vilber and Pop-Bio.
It’s also compatible with all industry-standard image formats:
.TIF/.TIFF
Phoretix 1D supports 8-bit, 16-bit (recommended) and higher uncompressed TIFF images. Multi-page and multiplex TIFFs are supported and each page is interpreted as a “channel” (all of which must have the same dimensions and pixel format).
.JPG/.JPEG/.PNG/.BMP/.IMG
While not recommended because of their low bit-depth and (sometimes) compression artifacts, these image formats can still be analyzed.
This means you aren’t locked in or reliant on one particular equipment manufacturer, reducing your business risk, allowing you greater freedom in your imaging equipment selection and facilitating easier tech transfer between labs.
Here at TotalLab we’ve been working with pharmaceutical customers for decades and we know that some of our users prefer using air-gapped computers to enhance security within their sites. This isn’t a problem, all TotalLab software can be activated easily either online or offline.
Our licensing system licenses a whole computer at once, with no restrictions on the number of users who have access to the software on that computer.
Licenses can be purchased singularly for one computer or we also offer discounts on multiple license purchases at the same time if you want to cover multiple computers across your site; enabling hybrid working to perform analysis outside of the lab or to allow multiple analyses to happen at the same time.
We can also support enterprise licensing for full site-wide licenses covering large pharmaceutical manufacturing facilities and research facilities at a significant discount vs buying singular or multiple licenses.
For pricing and any other licensing related queries please contact us.
Phoretix 1D, along with all TotalLab software, can be integrated into our 21 CFR Part 11/GMP-compliant AuditSafe software to generate a full audit trail with electronic signatures during analysis for use in regulated industry environments where data traceability, accountability, and integrity are critical.
AuditSafe restricts software and data access through secure user sign-ins, granular permissions, audit logs, electronic signatures and image authenticity checks. Click here to find out more.
The best way to ensure you’re purchasing the latest version of Phoretix 1D with the highest level of technical support is directly from us here at TotalLab by using the contact us form. We can accept payment in GBP (£), USD ($) or EUR (€).
We do appreciate that some of our customers prefer sourcing products locally in a local currency or prefer to deal in their native language which may not be English. To facilitate this, TotalLab work with a global network of authorized distributors which you can find here.
Here at TotalLab, we are a mixture of PhD-educated life scientists, mathematicians and software developers with decades of experience and we take pride in offering the highest level of support and educational resources to all of our customers. Our team of experts are dedicated to ensuring that you have the best experience possible with our software and we fully comprehend the scientific principles behind both your images and experiments.
We never use call centers – when you need support, be it through email or phone, you’ll speak directly to a member of the TotalLab team responsible for the development of our software. We understand your time is valuable, which is why we aim to resolve your queries as quickly as possible, ensuring minimal disruption to your workflow.
In addition to our exceptional support, we also offer a range of educational resources to help you make the most of our software. Our extensive library of tutorials and guides will provide you with the knowledge you need to use our software to its fullest potential. Whether you are a seasoned professional or a newcomer to our software, our resources will ensure that you have the skills you need to succeed.
You can access our video tutorials here:
Aftersales support
When you purchase from TotalLab, not only do you get access to our industry-leading advanced image analysis software but you also benefit from unlimited technical support, training and free updates to the latest version of your software on release.
If you have a question about using our software, please check our FAQ or Resources. Alternatively, you can email support@totallab.com and a member of our team will be in touch to help you.
Good image capture is critical to guarantee optimal performance of automated image analysis packages and generate reliable scientific data. This technical document provides a brief guide to the range of different image acquisition devices currently in use for gel applications, defines some the important technical factors required to generate digital images of a quality suitable for automated image analysis and recommendations for producing good 2D gel images.
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Learn how to read Western blot results with easy, actionable steps. From sample preparation to data analysis, TotalLab's guide covers everything you need for accurate protein detection. Perfect for researchers and lab professionals!
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In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.
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The automatic analysis features built into Phoretix 1D can save you huge amounts of time in a high-throughput environment and minimise inter-operator and inter-lab variability.
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The best methods for troubleshooting Western blots, including solutions for common issues like no signal, high background, non-specific bands, and uneven bands. Improve your Western blotting results with our detailed guide and visual aids
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