CLIQS - 1D gel and Western blot image analysis software

Accurate and reliable results of 1D gel and Western blots

Our CLIQS software enables you to analyse separation and quantification of your DNA, RNA and protein samples including multiplex Western blots.

The ability of CLIQS to compare lanes across 1D gel images is one of the software’s core features. It delivers absolute measurements rather than relative quantities and also enables colony counting and basic 2D spot measurement.

We liaise closely with our customers and industry to ensure that software features are kept up to date, meet current requirements and support ways of working. CLIQS’ flexibility and ability to function on a variety of devices means it can be used to analyse all common images files used for multiplexing. Enabling you to maximise the potential of the image capture instruments already in use in your lab environment.

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Collaborate on reporting image analysis results

Analysis and reporting of results is supported through the software’s 3D view, which enables a better visual of spot intensity when determining spots. Up to 1536 spots can be determined with its automatic and manual grid detection.

Image annotation, sharing  reports via Excel data tables and PDF analysis reports are all quick and easy to do. And the production of publication quality images is supported through Clip Gallery.

Dendrograms can be created to illustrate the hierarchical relationship between results, helping to indicate weak unions and the mean of each cluster.

To explore how CLIQS can be used in your environment contact us for a trial.

The benefits of audit trails in analysis

Our clients often ask for our support in helping them become 21 CFR part 11 compliant. This is why we introduced our GxP Module  software which can integrate with our image analysis software.

Through the use of GxP Module, 1D gel analysis results are stored in a secure folder, with audit trails of all work. Security controls require usernames and passwords, with user activity tracked, as well as password resets and fails. Electronic Signatures are also required for signing of work – which are then also stored for audit purposes.

Analysis of our Western Blots was performed in approximately 30 seconds using the fully automated process. The program is easy to navigate; analyses were performed with confidence by myself and by graduate students after a five minute demonstration.

Dr Roz Jenkins
- Principal Experimental Officer, Molecular and Clinical Pharmacology, Institute of Translational Medicine, University of Liverpool

Get in touch to trial CLIQS >

Download our CLIQS factsheet

CLIQS Factsheet

Why does automatic lane creation fail on my gel?

There could be several reasons why this happens…

  • The lanes are short or have very few bands
  • The lanes are too close together so the algorithm cannot split them
  • The lanes are not quite the same width which is an assumption the algorithm uses
  • The resolution of the image is very low and there is not enough band information

Using an AOI for lane detection can help (draw a box when in automatic detection mode) or simply try to create the lanes manually.

My image does not look like the image I captured. Why?

A histogram of all pixel values is created. Then the default auto contrast is created from the value of the histogram after 0.5% of all pixels and at 99.5% of all pixels. This stops outliers or scanner noise affecting the result but it can look a little different.

If the image has a calibration (from Powerscan) or an encoding (from GE or Fuji scanners) then the converted values are used by default for the image. If you do not want this then use the “Raw” option to go back to the raw pixels values.

Note: Contrast does not affect the values used in any calculations.

My question isn't listed here, where should I go?

We have more answered questions on our FAQ page here and likewise to our resources page where you may find the answer you need. If you’re still unsure you can always contact our support desksupport@totallab.com.