Streamline Data Analysis for Microarrays and Plate-Based Assays
Phoretix Array represents the latest evolution of our array assay-based image analysis software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time.
It offers users the ability to quickly and easily define a square grid of wells for quantification in experiments based within an array/plate format. Once defined, the user has a number of options to remove background “noise” from wells for accurate quantitation, define negative and positive controls, normalize their wells, perform relative quantity calibration and automatically mark wells as present or absent.
In the words of the papers and the forums: quantify the spots on a dot blot, cytokine array membrane, ELISpot plate or slot blot, subtract the background, normalize to your control spots, and do it the same way on the next membrane. You fit the grid to your array layout once, Phoretix Array saves it as a template, and the next image goes straight to a results table. If you are working out how to read dot blot results by eye, this is the step after that.
How to report Phoretix Array in your methods: “Spot intensities were quantified using Phoretix Array (TotalLab Ltd, Newcastle upon Tyne, UK) after background subtraction and normalized to the [reference/positive control] spots.” Replace the bracketed part with the controls on your membrane.
Phoretix Array is the complete solution for analyzing array-based experiments, it offers:
- Universal Compatibility: Compatible with fluorescence, chemiluminescence and colorimetric detection methods. Supports all image formats, including TIFF, IMG, JPEG, PNG and BMP files.
- Speed. Automate your entire analysis and get results in less than a minute per image.
- Objectivity. Unmatched automation reduces the need for manual intervention and minimizes inter-operator variability.
- Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
- Quantitation. Enables reliable, accurate quantitation of plate images.
- 21 CFR or GMP-compliance. When combined with our AuditSafe software, allows users to meet and even exceed FDA 21 CFR part 11/EudraLex Annex 11/GAMP5 standards for compliance within regulated or QC environments, from captured image all the way through to final analysis and report production.

Phoretix Array is compatible with the latest versions of Windows (currently Windows 11), macOS operating systems (up to and including Ventura and M1/M2/M3 and M4 processors) and Linux.

Our Phoretix Array software is trusted by the world’s leading academic and pharmaceutical companies:
