PlateLogic: Automated ELISA and Plate-Based Assay Analysis Software

The analysis layer for every plate reader in your lab.

PlateLogic is automated analysis software for ELISA and other plate-based assays. It takes the raw .CSV output from any plate reader and turns it into validated, audit-ready results, without the slow, inflexible software bundled with your instruments, and without the error-prone spreadsheets that usually fill the gap. Built in collaboration with scientists at the world’s leading pharmaceutical companies, PlateLogic is available in a 21 CFR Part 11 and GMP-compliant configuration for regulated QC and manufacturing environments.

A Modern Alternative to Legacy Plate Reader Software

ELISA remains one of the most widely used quantitative techniques in the pharmaceutical industry, yet the software scientists rely on to analyze it has barely changed in two decades. Most bench scientists, method owners and QC analysts will recognize these frustrations:

• Slow, dated software. The gold-standard analysis tools shipped with plate readers are difficult to drive, lag behind current operating systems, and are painfully slow to process results.
• No visibility across runs. Legacy packages treat every experiment in isolation. If you want to track how your standards, controls or samples are drifting over time, that work falls to you, in Excel, by hand.
• Manual, repetitive analysis. Despite the maturity of ELISA as a technique, analyzing the data is still laborious. Scientists spend hours on calculations that should be automated.
• Compliance burden. In regulated environments, every manual step is a risk, a data integrity gap, and a potential audit finding.

PlateLogic was designed from the ground up to solve all four problems at once.

Works with Every Plate Reader in Your Lab

PlateLogic doesn’t replace your plate reader software, it replaces the analysis work that comes after it. PlateLogic accepts the industry-standard .CSV export available from virtually every plate reader on the market, so you can keep your existing hardware, add new instruments as your lab evolves, and consolidate the analysis step onto a single platform across every site.

This vendor-neutral approach reduces capital expenditure, cuts the training burden of supporting multiple OEM analysis tools, and avoids the compatibility headaches that come with instruments acquired through mergers and acquisitions. One analysis workflow, every plate reader, every site.

Standardize Analysis Across Sites and Teams

PlateLogic enables fully customizable, automated analysis through node-based mathematical processing templates. Once a template is configured for a given assay, the analyst simply loads the template, imports the .CSV, and goes straight to validated results. No manual intervention, no operator interpretation, no spreadsheet gymnastics.

Templates can include:

• 4PL and 5PL curve fitting with automated goodness-of-fit checks
• Blank subtraction, replicate averaging, and outlier detection
• User-defined acceptance criteria and QC flags
• Automatic interpolation of unknowns from the standard curve
• Multi-plate analysis and batch processing
• Formatted, audit-ready report generation

Users can build an unlimited library of templates covering every routine assay in the lab, then share them across teams, sites or CDMO partners by email attachment. Everyone runs exactly the same analysis, exactly the same way, every time.

That removes operator error, collapses inter-operator variability, and harmonizes results across global production and research networks. It is particularly valuable for method transfer to CDMOs/CMOs, for comparability studies, and for any scenario where reproducibility across geographies is a regulatory or commercial requirement.

Track Long-Term Trends and Predict Failures Before They Happen

PlateLogic integrates seamlessly with TrendLab, TotalLab’s longitudinal QC trending platform. Together, they give method owners and QC managers a lab-wide view of how assays, standards and controls are performing over weeks, months and years, with predictive alerts before measurements breach their limits.

This is the kind of proactive data integrity oversight that legacy plate reader software simply cannot offer.

21 CFR Part 11 and GMP Compliance with AuditSafe

For regulated environments, PlateLogic integrates natively with AuditSafe, TotalLab’s 21 CFR Part 11 and GMP compliance module. Together they deliver full compliance with FDA 21 CFR Part 11, EudraLex Annex 11, GAMP 5, and ALCOA+ data integrity principles, from the exported .CSV file all the way through to final report production.

AuditSafe adds:

• Secure user authentication with role-based granular permissions
• Tamper-evident audit logs covering every action, user, timestamp and change reason
• Electronic signatures compliant with 21 CFR Part 11 Subpart C
• File authenticity and integrity verification
• Centralized configuration across multiple workstations and sites

For pharma QA leads, the combination means PlateLogic can be deployed in regulated QC labs, manufacturing environments, and clinical development workflows without a separate compliance layer.

Full IQ/OQ Validation Packages Available

TotalLab has supported the world’s largest pharmaceutical companies with production-ready, validated software for over a decade. We provide a complete in-person IQ/OQ package to accompany every regulated deployment of PlateLogic, removing validation effort from your in-house team and accelerating time-to-use in GMP environments.

Flexible Licensing Options

We understand that pharmaceutical and academic labs have very different IT and security requirements. PlateLogic supports both:

• Online or offline activation, for sites with restricted network access
• Per-computer licensing with unlimited users on each licensed machine
• Multi-license discounts for labs deploying PlateLogic across multiple workstations
• Enterprise site licenses for large pharmaceutical manufacturing and research facilities, at significant volume discounts

For hybrid working, out-of-lab analysis, or parallelized workflows, multiple licenses allow your team to work concurrently without bottlenecks.

For pricing and licensing queries, please contact us.

How to Purchase PlateLogic

The best way to secure the latest version of PlateLogic with the highest level of technical support is directly from TotalLab via our contact form. We accept payment in GBP (£), USD ($) and EUR (€).

We also work with a global network of authorized distributors for customers who prefer to source locally, in local currency, or in a native language. You can find your local distributor here.

Frequently Asked Questions

Is PlateLogic a SoftMax Pro alternative? Yes. PlateLogic is designed as a modern, vendor-neutral replacement for legacy plate reader software such as SoftMax Pro, Magellan and Gen5. Unlike vendor-locked tools that only work with one manufacturer’s readers, PlateLogic accepts .CSV exports from virtually any plate reader, allowing you to consolidate analysis across every instrument in your lab onto a single platform.

Does PlateLogic control my plate reader? No. PlateLogic is analysis software. You continue to run your plate reader using whatever software it shipped with, then export the results as a .CSV file and open them in PlateLogic for analysis. This is a deliberate design choice: by focusing purely on analysis, PlateLogic works with every plate reader brand on the market and can be deployed without IT changes, instrument revalidation, or capital expenditure on new hardware.

What assays does PlateLogic support? PlateLogic is built for ELISA (sandwich, direct, indirect, competitive), HCP ELISA, and a wide range of other plate-based assays including absorbance, fluorescence and luminescence readouts, AlphaLISA, MSD, cell-based potency assays, and endpoint or kinetic experiments. If it runs on a microplate and exports to .CSV, PlateLogic can analyse it.

Is PlateLogic 21 CFR Part 11 compliant? Yes, when deployed with TotalLab’s AuditSafe compliance module. Together they deliver full compliance with FDA 21 CFR Part 11, EudraLex Annex 11, GAMP 5 and ALCOA+, including audit trails, electronic signatures, user access controls and data integrity verification. A full IQ/OQ validation package is included for regulated deployments.

Does PlateLogic support 4PL and 5PL curve fitting? Yes. PlateLogic includes robust 4-parameter and 5-parameter logistic curve fitting, alongside linear, quadratic, point-to-point and other common fit models, with automated goodness-of-fit checks, residual analysis and outlier detection.

Can I use PlateLogic with my existing plate reader? Yes. PlateLogic is analysis software, not reader control software, which means it works alongside your existing plate reader rather than replacing it. It accepts .CSV exports from the full range of major plate reader brands, including Molecular Devices, Tecan, BMG LABTECH, BioTek, Thermo Fisher and others. You keep using your instrument exactly as you do today; PlateLogic takes over once the data is exported.

How does PlateLogic handle method transfer between sites? Analysis templates can be built once and shared across teams, sites or CDMOs as simple file attachments. Every lab using the same template runs exactly the same analysis, eliminating inter-site variability, simplifying comparability studies, and de-risking method transfer.

Is there a free trial or demo available? Yes. Contact us to arrange a free demonstration with one of our product specialists, who can walk you through PlateLogic using your own assay data.

Resources