SpotMap - host cell protein coverage analysis software

A complete solution for analysing HCP coverage with 2D gel electrophoresis and Western blot images

Our SpotMap software first launched to market in 2015 and is the industry-leading software for host cell protein (HCP) antibody coverage analysis in the pharmaceutical industry.

SpotMap is a complete solution for analysing HCP coverage with 2D gel electrophoresis and Western blot images.

Speed. Get a coverage % in less than 10 minutes.
Objectivity. Unmatched automation reduces the need for manual intervention.
Reproducible. Evidence of consistent coverage results between different users and labs.
Quantitation. Enables reliable, accurate quantitation of gel and blot images.
21 CFR ready. Meet 21 CFR part 11/EU Annex 11 compliance for GMP/GLP labs from captured image to final analysis.

SpotMap solves the common problems you face in 2D gel electrophoresis vs Western blot analysis for HCP coverage such as no reproducibility in results between users, hours of unnecessary spot matching and a lack of confidence in the percentage coverage results.

We work closely with the biopharma industry and act on feedback to continuously ensure that our SpotMap software enables users to monitor HCP impurities and reach their coverage requirements to enable their drug-to-market journey.

Our SpotMap software is trusted by the world’s leading pharmaceutical companies

"SpotMap played an integral role in our comparison of proteomes of different cell lines during the process of choosing a proper antigen for antibody development. The ability to overlay gels and assign numbers to each protein spot was especially useful in determining similarities and differences between gels without duplicating identified spots. One feature that I found to be especially useful was the 3D view which allowed for a better visual of spot intensity when determining spots. The table of identified spot for each image also made it easy to identify which spots are unique or common to the different images. With this software, my team was able to make a more informed choice on the best candidate to use for our purposes."

Virginia Liu-Compton
- Senior Scientist II, ImmunoGen Inc, Waltham, Massachusetts, USA

Optimise your host cell protein analysis workflow

We use intuitive algorithms, and easy-to-use interface, to enable users to obtain an accurate picture of coverage in around 10 minutes.

This – combined with advanced automation – enables users to achieve extremely reproducible antibody-to-host cell protein coverage, even between different users and labs. It provides reliable, accurate quantitation of gel and blot images so that you can achieve objective results.

Users are also able to verify host cell protein antibody reactivity, giving you a more complete understanding of the antigen quality.

If you’re looking for software to support HCP coverage analysis, make bioprocess improvements or achieve analytical development then request a free demo of SpotMap. You’ll receive guidance on how to achieve reproducible results, tips for aligning challenging gels and western blots and knowledge on the software tools to use on more challenging images, such as faint spots.

SpotMap played an integral role in our comparison of proteomes of different cell lines during the process of choosing a proper antigen for antibody development. The ability to overlay gels and assign numbers to each protein spot was especially useful in determining similarities and differences between gels without duplicating identified spots.

Industry User

Report with confidence

Using our software you can view your data from a variety of perspectives with efficiency, investigate and visualise any suspected cross-reactivity and future proof your analysis results with our GxP Module integration.

21 CFR part 11 regulations stipulate the need to track, record and authenticate your experiments. With our GxP Module software as an add-on, you’re able to monitor system access, record user activity and ensure compliance with FDA regulation. You can read more here.

Download our guide to SpotMap

How can I ensure the quantitative accuracy of my HCP coverage analysis using SpotMap?

In this video we show you how automatic spot outlines in SpotMap obtain an objective, accurate and quantitative analysis and how you can use 3D mode to validate the software you currently use for HCP coverage.

How do I use Image QC in SpotMap?

Image QC is used to quality check any images added to your project, before you run analysis. This helps to save time as any issues with the image are immediately highlighted, for you to address before any further analysis is attempted.

Image QC flags any potential issues with:

Bit-depth
Image file size
Saturation
Manual editing of the images

Poor image quality can make analysis of your gels and blots difficult and may reduce the objectivity of your analysis.

My question isn't listed here, where should I go?

We have more answered questions on our FAQ page here and likewise to our resources page where you may find the answer you need. If you’re still unsure you can always contact our support desk, support@totallab.com.