CLIQS 1D Pro Image Database Software | Tutorial Series | How to Match Bands Across Your Database

In this video we show you how to perform a search of your blot or gel database to find matching bands across lanes, how to fine-tune your search parameters and how to express your matches statistically.

This is especially useful if you have a known set of standards for QC of gels/blots that you wish to run against your database to make sure they’re present in every experiment or wish to run a longitudinal experiment and want to identify when known proteins appear over time etc.

00:00 – Intro

00:18 – Display Explanation

00:35 – Match Parameters Explained

00:45 – Match Sets

01:47 – RF Vector Explained

02:17 – Minimum and Maximum RF Explained

02:47 – Lane Similarity Algorithm

03:13 – Band Classes

05:17 – Profile Display Options

05:48 – Show Molecular Size

05:55 – Remove Background

06:36 – Table Settings

07:43 – Adding and Removing Bands

08:27 – Matched Bands

08:47 – Adding and Removing Matches

09:16 – Lane Pairs

10:31 – Band Matrix

12:18 – Lane Similarity

13:02 – Lane Similarity Score

13:55 – Exporting Data

14:34 – Outro

CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots which can then be searched and compared to unknown samples to quickly identify them. The algorithms embedded within CLIQS 1D Pro effectively compensate for any distortions between different gel runs to allow accurate comparisons and matching between samples, as if they were all run on the same gel at the same time.

CLIQS 1D Pro was specifically designed to facilitate research techniques such as Inter simple sequence repeat (ISSR)-PCR, PFGE bacterial DNA fingerprinting (PFGE), denaturing gradient gel electrophoresis (DDGE), restriction fragment length polymorphism, enterobacterial repetitive Intergenic Consensus (ERIC) PCR and single-strand conformation polymorphism (SSCP) electrophoresis.

For a free trial of CLIQS 1D Pro to see how well it works creating a database from your own images, please fill in the form here.