Phoretix 1D - advanced image analysis

Advanced 1D gel and Western blot image analysis software

Phoretix 1D is the latest evolution of our 1D software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time.

We have distilled our twenty years’ worth of experience supporting the life science industry into Phoretix 1D to deliver our most advanced set of analysis tools yet.

Phoretix 1D offers automatic lane and band detection or, for even deeper automation and standardisation of your workflow, user-defined protocols which allow full one-click analysis of gel or Western blot images. This removes the potential for operator error entirely.

Coupled with intuitive background removal using industry-leading algorithms, molecular weight calculation, quantity calibration and normalisation Phoretix 1D has all the modern tools required for comprehensive experimental analysis.

Our software is device agnostic, so can help reduce costs as it can be used with your existing laboratory equipment, it also works with all industry-standard image formats (.tif, .tiff, .gel, .img, .jpg, .jpeg, .bmp and .png) and is compatible with the latest versions of both Windows and macOS operating systems (11 and Monterey).

Get in touch for a product demo

Intuitive detection and quantification of gel electrophoresis data

As with all our software, Phoretix 1D has been designed with an intuitive and easy-to-use interface to minimise the time required to train new operators. Our simplified workflow guides the user through their gel electrophoresis and blotting analysis whilst leaving a large, central viewing area of the image and data being analysed.

If you’d like a demo to see just how easy it is to use, please get in touch.

automatic band detection mode for immunoblots and electrophoresis gels

Supporting 21 CFR Part 11 compliance in GxP laboratories / environments

Phoretix 1D, along with all TotalLab software, can be integrated into our 21 CFR Part 11 compliant GxP Module for regulated environments where data traceability, accountability, and integrity are critical.

Our GxP Module features access control through secure sign-ins, audit logs, electronic signatures and image authenticity checks. Click here to find out more.

Aftersales support

When you purchase from TotalLab, not only do you get access to our industry-leading advanced image analysis software but you also benefit from unlimited technical support, training and free updates to the latest version of your software on release, all included within your subscription.

If you have a question about using our software, please check our FAQ or Resources. Alternatively, you can email and a member of our team will be in touch to help you.

Get in touch to trial Phoretix 1D >

How can I analyse a Western blot?

Phoretix 1D is our latest offering to the life science sector for analysing 1D gels and Western blots. In this tutorial series we provide an overview in the platform – watch here. And get in touch for a free trial here.

Why does automatic lane creation fail on my gel?

There could be several reasons why this happens…

  • The lanes are short or have very few bands
  • The lanes are too close together so the algorithm cannot split them
  • The lanes are not quite the same width which is an assumption the algorithm uses
  • The resolution of the image is very low and there is not enough band information

Using an AOI for lane detection can help (draw a box when in automatic detection mode) or simply try to create the lanes manually.

My question isn't listed here, where should I go?

We have more answered questions on our FAQ page here and likewise on our resources page where you may find the answer you need. If you’re still unsure you can always contact our support