Phoretix 1D - DNA/RNA Gel and Western Blot Analysis Software

Automatic Gel Electrophoresis and Immunoblot Analysis Software

Phoretix 1D is the latest evolution of our 1D software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time.

We have distilled our twenty years’ worth of experience supporting the life science industry into Phoretix 1D to deliver our most advanced set of analysis tools yet to analyse images derived from any Gel Documentation, densitometer or other imaging devices.

Phoretix 1D is the complete solution for analysing gel electrophoresis and immunoblot images, it offers:

    • Speed. Automate your entire analysis and get results in less than a minute per image.
    • Objectivity. Unmatched automation reduces the need for manual intervention and minimises inter-operator variability.
    • Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
    • Quantitation. Enables reliable, accurate quantitation of gel and blot images.
    • 21 CFR/GMP-compliant Meet FDA 21 CFR part 11/EU Annex 11/GAMP5 GMP compliance for regulated environments, from captured image through to final analysis and report production.

Phoretix 1D is compatible with the latest versions of both Windows (currently Windows 11) and macOS operating systems (up to and including Ventura and M1/M2 processors).

Our Phoretix 1D software is trusted by the world’s leading pharmaceutical companies

Get your free trial now!

Automatic detection and quantification of gel electrophoresis data

Phoretix 1D offers automatic lane and band detection or, for even deeper automation and standardisation of your workflow, user-defined protocols which allow full one-click analysis of gel or Western blot images. This removes the potential for operator error entirely.

Coupled with intuitive background removal using industry-leading algorithms, molecular weight calculation, quantity calibration and normalisation Phoretix 1D has all the modern tools required for comprehensive experimental analysis.

automatic band detection mode for Western Blot and electrophoresis gels
Western/Immunoblot background subtraction

Advanced background removal and noise correction

Background noise can come from various sources, such as non-specific binding of antibodies or stains, uneven illumination, dust, scratches, or instrument artifacts. If not corrected, background noise can affect the intensity and shape of the bands or spots, leading to inaccurate measurements of their size, quantity, and relative expression. Therefore, it is important to perform background removal to subtract the unwanted signal from the image, before quantifying bands or spots.

Our Phoretix 1D software features multiple methods of background removal for our users to choose from and refine their analysis:

Adaptive

  • Rolling Ball
  • Lane Edge
  • Rubber Band

User-Defined

  • None
  • Constant
  • Image Rectangle
  • Profile Minimum

It also features innovative noise-reduction techniques to smooth out the intensity signal of your bands or spots, resulting in cleaner data for analysis.

You can find out more about our background removal options here or watch the video below.

"Phoretix 1D allows for the fast and accurate analysis of immunoblots, supported by optimal control over quantification parameters allowing for the generation of highly reproducible data. All features are embedded within a user-friendly interface, I especially liked the background analysis features which assist in an unbiased analysis by correcting for irregular background signals."

Jacqueline Kaiser, The University of Melbourne

Analyse a huge variety of experiments

Phoretix 1D is the perfect choice for the quantitative analysis of:

  • DNA/RNA Gels
  • Western Blots
  • Northern Blots
  • Southern Blots
  • Thin-layer chromatography
  • Lateral flow tests (LFT/LFA)
3D view of electrophoresis gel
automatic band detection mode for Western Blot and electrophoresis gels

Harmonise your lab equipment

Our software is device agnostic, so can reduce costs as it can be used with all of your existing laboratory equipment or any additional equipment gained during business acquisitions, it also works with all industry-standard image formats:

.TIF/.TIFF

Phoretix 1D supports 8-bit, 16-bit (recommended) and higher uncompressed TIFF images. Multi-page and multiplex TIFFs are supported and each page is interpreted as a “channel” (all of which must have the same dimensions and pixel format).

.JPG/.JPEG/.PNG/.BMP/.IMG

While not recommended because of their low bit-depth and (sometimes) compression artifacts, these image formats can still be analysed.

This means you aren’t locked in or reliant on one particular equipment manufacturer, reducing your business risk, allowing you greater freedom in your imaging equipment selection and facilitating easier tech transfer between labs.

Supporting 21 CFR Part 11 compliance in GxP laboratories/environments

Phoretix 1D, along with all TotalLab software, can be integrated into our 21 CFR Part 11/GMP-compliant AuditSafe software to generate a full audit trail with electronic signatures during analysis for use in regulated industry environments where data traceability, accountability, and integrity are critical.

AuditSafe restricts software and data access through secure user sign-ins, granular permissions, 1audit logs, electronic signatures and image authenticity checks. Click here to find out more.

Supporting our customers

Here at TotalLab, we are a mixture of PhD-educated life scientists, mathematicians and software developers with decades of experience and we take pride in offering the highest level of support and educational resources to all of our customers. Our team of experts are dedicated to ensuring that you have the best experience possible with our software and we fully comprehend the scientific principles behind both your images and experiments.

We never use call centers – when you need support, be it through email or phone, you’ll speak directly to a member of the TotalLab team responsible for the development of our software. We understand your time is valuable, which is why we aim to resolve your queries as quickly as possible, ensuring minimal disruption to your workflow.

In addition to our exceptional support, we also offer a range of educational resources to help you make the most of our software. Our extensive library of tutorials and guides will provide you with the knowledge you need to use our software to its fullest potential. Whether you are a seasoned professional or a newcomer to our software, our resources will ensure that you have the skills you need to succeed.

You can access our video tutorials here:

Aftersales support

When you purchase from TotalLab, not only do you get access to our industry-leading advanced image analysis software but you also benefit from unlimited technical support, training and free updates to the latest version of your software on release.

If you have a question about using our software, please check our FAQ or Resources. Alternatively, you can email support@totallab.com and a member of our team will be in touch to help you.

Get in touch to trial Phoretix 1D >