How to Calculate Host Cell Protein (HCP) Antibody Coverage %
This tutorial will show you how to analyse 2D gel electrophoresis and western blot images using TotalLab’s SpotMap HCP image analysis software and calculate a percentage coverage score.
SpotMap is an industry-leading and complete solution for analysing HCP coverage using 2D gel electrophoresis and Western blot images.
0:00 – Intro
0:30 – How to Import Your Images
1:00 – Main Window Overview
1:40 – Contrast/Brightness Correction and False-Colour Overlays
2:50 – Image Editing Tools Within SpotMap
4:50 – Quality Control of Images
6:05 – MW Strip Calibration
06:55 – pI Strip Calibration
7:26 – Annotation of Spots
9:03 – How to Align Your Images
11:23 – Manual Alignment
14:58 – One-click Automatic Alignment
16:22 – Automatic Spot Detection
17:44 – Manual Spot Editing Tools
21:03 – Automatically Detecting New Spots
21:18 – Using Spot Sets
21:28 – Marking Spots as Present
21:52 – Marking Spots as Absent
23:01 – Filtering by Spot Intensity
24:14 – Calculating Coverage Percentage
25:05 – Automatic Spot Sets
25:57 – Defining Product Area
27:05 – Finding Significant Differences Between Spots
27:15 – Spot Measurements
28:05 – 3D View of Spots
29:00 – Exporting PDF Reports
31:00 – Example Report
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