Automated Array Data Analysis Software for ELISA, Dot Blots, and Microarrays | Phoretix Array

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Streamline Data Analysis for Microarrays and Plate-Based Assays

Phoretix Array represents the latest evolution of our array assay-based image analysis software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time.

It offers users the ability to quickly and easily define a square grid of wells for quantification in experiments based within an array/plate format. Once defined, the user has a number of options to remove background “noise” from wells for accurate quantitation, define negative and positive controls, normalize their wells, perform relative quantity calibration and automatically mark wells as present or absent.

Phoretix Array is the complete solution for analyzing array-based experiments, it offers:

  • Universal Compatibility:  Compatible with fluorescence, chemiluminescence and colorimetric detection methods. Supports all image formats, including TIFF, IMG, JPEG, PNG and BMP files.
  • Speed. Automate your entire analysis and get results in less than a minute per image.
  • Objectivity. Unmatched automation reduces the need for manual intervention and minimizes inter-operator variability.
  • Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
  • Quantitation. Enables reliable, accurate quantitation of plate images.
  • 21 CFR or GMP-compliance. When combined with our AuditSafe software, allows users to meet and even exceed FDA 21 CFR part 11/EudraLex Annex 11/GAMP5 standards for compliance within regulated or QC environments, from captured image all the way through to final analysis and report production.
    TotalLab software is fully compliant with FDA 21 CFR Part 11, EU Annex 11, and GMP regulations, ensuring regulatory readiness for pharmaceutical and life science applications.

Phoretix Array is compatible with the latest versions of Windows (currently Windows 11), macOS operating systems (up to and including Ventura and M1/M2/M3 and M4 processors) and Linux.

Our Phoretix Array software is trusted by the world’s leading academic and pharmaceutical companies:

Phoretix Array DNA/RNA Microarray analysis

Why Choose Phoretix Array?

We have distilled our decades of experience into our Phoretix Array automated array analysis software, which has been developed in close collaboration with the pharmaceutical industries needs and feedback to deliver the highest-quality data in the shortest amount of time with the lowest inter-operator variability possible.

Key Features:

  • Universal Compatibility:  Compatible with fluorescence, chemiluminescence and colorimetric detection methods. Supports all industry-standard scanners and image formats, including TIFF, IMG, JPEG, PNG and BMP files.
  • Accuracy You Can Trust. All measurements are taken directly from the raw image – no compression, no scaling. Calculations are based on peer-reviewed methods, tested with a comprehensive suite of automated and manual validations.
  • Pre-configured Templates. Phoretix Array comes pre-configured with the industry standard 96 and 384 well plate templates – simply click and drag to position your measurement grid over your image.
  • Comprehensive Background Subtraction. Phoretix Array has a range of background subtraction methods for users to choose from, allowing accurate results from even the noisiest images
  • Normalization. Phoretix Array allows users to normalize their data either to a specific well within their experiment or to the average value of multiple wells combined together.
  • Quantity Calibration. Using known standards in an experiment, Phoretix Array can perform accurate quantification for unknown wells within an experiment.
  • Presence Assignment. With Phoretix Array, users can define a threshold for presence/absence calculations to quickly identify positive and negative sample results across the whole array.
  • Results Export. Export your results as either a .PDF report or a raw .CSV file for further calculation.

Who Is It For?

Phoretix Array is ideal for researchers who:

  • Process large volumes of array or plate-based assays
  • Need consistent and automated processing of their array or plate data
  • Value reproducibility in their workflow
  • Are frustrated with fragmented workflows in heavily manual tools like ImageJ and Excel
  • Want standard, modern usability functionality like undo and redo that are frequently missing in other outdated scientific tools for array analysis

Don’t take our word for it, download your free trial today to see how Phoretix Array works with your images, from your own lab.

ELIFA Image Analysis
ELIFA 3D View
Phoretix Array ELIFA Measurements

Multiple experiments, one piece of analysis software

Phoretix Array is ideal for the quantitative analysis of:

  • ELISpot assays
  • FluoroSpot assays
  • Dot blots for cytokine analysis
  • Slot blots
  • HLA Typing
  • Enzyme-linked immunosorbent assay (ELISA) assays
  • Enzyme-linked immunofiltration Assay (ELIFA) assays
  • In Cell Western Blots
  • Microtiter plates
  • DNA or RNA microarrays
  • Zebrafish assays in plates

This coverage makes Phoretix Array highly relevant for research and clinical labs performing plate-based and array-based experiments, providing reliable, reproducible quantification and streamlined data workflows.

As with all our software, Phoretix Array has been designed with an intuitive and easy-to-use interface to minimize the time required to train new operators. Our simplified workflow guides the user through their analysis whilst leaving a large, central viewing area of the image and data being analyzed.

If you’d like a demo to see just how easy it is to use, please get in touch.

One-Click Quantification and Collaboration

Phoretix Array offers users incredibly customizable 1-click automatic analysis of array-based images. Whilst you’re working on your analysis, Phoretix Array intelligently captures your analysis settings and allows you to save them as a template file to be used again on your next experiment. When it’s time to analyze your next array simply load the previous template and go straight to your results with no need for manual intervention, saving users a huge amount of manual labor and, importantly, time.

A user can build up an unlimited library of templates to cover all their routine analyses and then share them easily sent with colleagues or even other sites anywhere in the world via something as easy as an email attachment to ensure they’re all running exactly the same analysis. This removes the potential for operator error entirely, greatly reduces inter-operator variability therefore reducing risk, harmonizing multiple global production or research sites and for tech transfer to a CDMO/CMO.

21 CFR Part 11 and GMP/GMP-compliance

SpotMap MS, along with all TotalLab software, can be integrated into our 21 CFR Part 11/GMP-compliant AuditSafe software to ensure full 21 CFR/ALCOA/GMP/EU Annex 11 compliance for use in regulated industry environments (such as the pharmaceutical industry) where data traceability, accountability, and integrity are critical.

AuditSafe restricts software and data access through secure user sign-ins, granular permissions, audit logs, electronic signatures and image authenticity checks. Click here to find out more.

Full IQ/OQ Validation Packages Available

At TotalLab, we’ve been supporting the world’s largest pharmaceutical companies with highly secure, production-ready versions of our software for over a decade now and enjoy very good relationships across the industry.

We know the importance of properly validated software and how it forms part of your QC journey so we offer a full, in-person IQ/OQ package to accompany the sale of regulated versions of our software

21 CFR Compliance

Flexible Licensing Options

At TotalLab we’ve been working with pharmaceutical and academic life science customers for decades, we know that some of our users prefer offline licensing to enhance security within their sites. This isn’t a problem, all TotalLab software can be activated easily either online or offline.

Our licensing system licenses a whole computer at once, with no restrictions on the number of users who have access to the software on that computer.

Licenses can be purchased singularly for one computer or we also offer discounts on multiple license purchases at the same time if you want to cover multiple computers across your site; enabling hybrid working to perform analysis outside of the lab or to allow multiple analyses to happen at the same time.

We can also support enterprise licensing for full site-wide licenses covering large pharmaceutical manufacturing facilities and research facilities at a significant discount vs buying singular or multiple licenses.

For pricing and any other licensing related queries please contact us.

At TotalLab, we’re the trusted partner of some of the world’s largest pharmaceutical companies, so we understand the importance of support and maintenance contracts for software, especially in time-critical and high-value processes.

We take pride in offering the highest level of support and educational resources to all of our customers. Our team of PhD-educated life science experts are dedicated to ensuring that you have the best experience possible all whilst understanding the scientific principles behind your experiments. Pricing for unlimited technical support, training and free updates to the software are included in your license cost – one fee, complete piece of mind. You’ll always be eligible for the latest version of the software on the latest operating systems so there’s no fear of future operating system incompatibility or running insecure, outdated software for critical operations.

We never use call centers or ticketing systems – when you need support you’ll speak directly to a member of the TotalLab team. We understand your time is valuable, which is why we aim to resolve your queries as quickly as possible, ensuring minimal disruption to your workflow.

In addition to our exceptional support, we also offer a range of educational resources. Our extensive library of tutorials and guides will provide you with the knowledge you need to use our software effectively. Whether you are a seasoned professional or a newcomer, our resources will ensure that you have the skills you need to succeed.

You can access our video tutorials here:

If you have a question about using our software, you can email support@totallab.com and a member of our team will be in touch to help you.

Resources

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