Pattern Matching & Dendrogram Generation in CLIQS 1D Pro

Pattern Matching & Dendrogram Generation in CLIQS 1D Pro: A Smarter Way to Analyze 1D Electrophoresis Data

In modern molecular biology and microbial typing, electrophoresis banding pattern analysis is a cornerstone technique. Whether you’re studying strain typing, clonal relationships, or genomic polymorphism, the need for a fast, objective, and reproducible method to compare fingerprint profiles across multiple samples is critical.

CLIQS 1D Pro, developed by TotalLab, offers an intelligent, streamlined approach to 1D gel electrophoresis band matching, database creation, and dendrogram generation — ideal for life science researchers, microbiologists, and quality control labs in pharma, biotech, and academia.

Why Band Matching Matters in 1D Gel Analysis

In many fields — from microbial epidemiology to plant genetics – researchers rely on 1D based electrophoresis techniques (such as SDS-PAGE, RAPD, RFLP, or PFGE) to generate unique banding patterns for different samples.

But comparing these patterns manually is slow, subjective, and difficult to scale effectively.

CLIQS 1D Pro solves this by offering:

• Accurate lane and band detection
• Automated band matching across multiple gels
• Database construction for long-term tracking and longitudinal studies
• Cluster analysis with dendrogram output

CLIQS 1D Pro is not about quantifying band intensity or normalizing simple gels and blots – we created Phoretix 1D for that purpose – it’s about identifying shared patterns and differences across large 1D-based datasets, quickly and reliably.

Build Robust Databases for Gel Pattern Comparison

CLIQS 1D Pro allows researchers to import, store, and organize electrophoresis data from multiple experiments into a centralized DNA fingerprint database.

Features include:

• Assign metadata to each lane/sample (e.g., strain ID, sample source)
• Group and subset samples for focused analysis
• Track fingerprints over time or across different experimental conditions

This structure makes it easy to compare historical data and perform targeted reanalysis.

🧠 Think of it as a relational gel database for molecular typing — no LIMS integration required.

Match Bands Across Gels Automatically

With CLIQS 1D Pro’s intelligent pattern recognition engine, you can:

• Automatically match bands across lanes and gels
• Define custom tolerance windows for migration differences
• Visualize matching vs. non-matching bands in a tabular or visual format
• Correct mismatches manually if required

CLIQS 1D Pro gives you full control over the matching algorithm – but without the manual tedium of aligning every band yourself.

As with all TotalLab software, CLIQS 1D Pro is compatible with with TIFF standard image format, making it fully vendor-neutral.

Generate Dendrograms for Clustering and Typing

Once your gel database is populated and band matches are assigned, CLIQS 1D Pro enables you to generate cluster trees (dendrograms) using established, industry standard statistical algorithms:

• UPGMA (Unweighted Pair Group Method with Arithmetic Mean)
• Dice or Jaccard coefficients for similarity scoring
• Flexible thresholding and group definition

Use dendrograms to:

• Detect strain similarity in microbial or viral populations
• Confirm clonal relatedness in outbreak tracking
• Group plant or animal varieties based on shared band features

🧬 This is ideal for researchers working in molecular epidemiology, vaccine QC, microbial fingerprinting, and evolutionary biology.

CLIQS 1D Pro vs. GelCompar II: Faster, Simpler, Just as Powerful

While tools like BioNumerics GelCompar II have dominated traditional gel analysis workflows, their steep learning curves and legacy UI are often barriers for modern labs.

CLIQS 1D Pro offers: