Guide
This tutorial will show you how to analyze 2D gel electrophoresis and western blot images using TotalLab’s SpotMap HCP image analysis software and calculate a percentage coverage score.
SpotMap is an industry-leading and complete solution for analyzing HCP coverage using 2D gel electrophoresis and Western blot images.
0:00 – Intro
0:30 – How to Import Your Images
1:00 – Main Window Overview
1:40 – Contrast/Brightness Correction and False-Color Overlays
2:50 – Image Editing Tools Within SpotMap
4:50 – Quality Control of Images
6:05 – MW Strip Calibration
06:55 – pI Strip Calibration
7:26 – Annotation of Spots
9:03 – How to Align Your Images
11:23 – Manual Alignment
14:58 – One-click Automatic Alignment
16:22 – Automatic Spot Detection
17:44 – Manual Spot Editing Tools
21:03 – Automatically Detecting New Spots
21:18 – Using Spot Sets
21:28 – Marking Spots as Present
21:52 – Marking Spots as Absent
23:01 – Filtering by Spot Intensity
24:14 – Calculating Coverage Percentage
25:05 – Automatic Spot Sets
25:57 – Defining Product Area
27:05 – Finding Significant Differences Between Spots
27:15 – Spot Measurements
28:05 – 3D View of Spots
29:00 – Exporting PDF Reports
31:00 – Example Report
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Keep reading
All SpotMap 2D resources →- GuideWhat is Host Cell Protein?Discover what host cell proteins (HCPs) are, why they matter in biologic drug development, and how they’re analyzed for safety. Learn about common HCP analysis methods, challenges, and strategies to manage risks, with insights on tools from Totallab
- GuideHCP ELISA vs LC-MS: Why Your ppm Numbers DisagreeAn HCP ELISA and an LC-MS assay measure different things, so their ppm values rarely agree on the same sample. This resource explains the five mechanisms behind the gap, walks through a reconciliation, and shows how SpotMap MS gives you an LC-MS number you can put beside the ELISA result in a filing.
- ComparisonHCP Antibody Coverage: 2D Western Blot vs AAE vs ELISA-MSExplore the current landscape of anti-HCP antibody coverage with our article comparing 2D DIGE, ELISA, and AAE. Optimize your antibody selection process for robust anti-HCP analysis in biopharmaceutical research.
SpotMap 2D2D western blot HCP antibody coverage analysis, reporting coverage overall and for each quadrant.