Guide
Here at TotalLab, we are frequently asked which of the background removal methods available in Phoretix 1D is the “correct” one to use.
In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.
If you are moving from another package and need to translate a rolling ball radius into the equivalent Phoretix 1D setting, use our Phoretix 1D rolling ball size converter.
00:00 – Introduction
00:22 – Explanation of What Background Is
02:00 – Rolling Ball Background Removal
03:42 – Rubber Band Background Removal
04:02 – Constant Value Background Removal
04:48 – Profile Min Background Removal
05:12 – Image Rectangle Background Removal
06:05 – How to Choose Between Different Background Removal Techniques
Phoretix 1D is also available in a fully 21 CFR Part 11 and EU Annex 11 compliant version for use in regulated industries such as medical device production.
For a free trial of Phoretix 1D to see how well it works with your images, please fill in the form here.
Phoretix 1D can provide quantitative analysis of blot and gel images, lateral flow test images, TLC (thin layer chromotography) images and more.
See our other image analysis-based products here.
Keep reading
All Phoretix 1D resources →- GuideHow to capture quantitative western blot and gel imagesHow to capture western blot and gel images you can quantify: 16-bit grayscale, the linear range and the two kinds of saturation, choosing the exposure, resolution, file formats and a 12-point checklist.
- GuideTotal protein normalization for western blotsHow total protein normalization works, how stain-free, Ponceau S and fluorescent total protein stains compare, how to capture a valid total protein image, and how to normalize to total lane volume in Phoretix 1D.
- ComparisonImage Studio Lite replacement: your options in 2026What replaced Image Studio Lite, and how Image Studio, Empiria Studio and Phoretix 1D compare on imagers, lanes, validation, platforms, Part 11 and price.
Phoretix 1DAutomated lane and band detection, densitometry and normalization for 1D gels and western blots.