Guide
Download our guide on the problems you can overcome when using our SpotMap software.
It covers key features, image QC, image analysis and details on the analysis element of the software.
Keep reading
All SpotMap 2D resources →- GuideWhat HCP Antibody Coverage Percentage Is Acceptable?There is no regulatory threshold for HCP antibody coverage. This resource traces where the 70 percent figure came from, explains why 2D gel and LC-MS coverage percentages are not comparable, and sets out what to state alongside any coverage claim, with SpotMap 2D producing the 2D gel coverage number you can defend.
- GuideHost Cell Protein Analysis: ELISA, 2D and LC-MS MethodsHost Cell Protein (HCP) impurity analysis is essential for safe biologics manufacturing. Discover how TotalLab’s software tools help to streamline HCP quantification when using gold-standard techniques like ELISA, 2D-PAGE, and LC-MS to support regulatory compliance and therapeutic safety.
- ComparisonHCP Antibody Coverage: 2D Western Blot vs AAE vs ELISA-MSExplore the current landscape of anti-HCP antibody coverage with our article comparing 2D DIGE, ELISA, and AAE. Optimize your antibody selection process for robust anti-HCP analysis in biopharmaceutical research.
SpotMap 2D2D western blot HCP antibody coverage analysis, reporting coverage overall and for each quadrant.

