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TotalLab and Merck Collaboration Streamlines 2D Gel Electrophoresis
By combining our industry leading SameSpots and SpotMap software with their Auto2D hardware, Merck have been able to rapidly reduce the time taken for complete 2D based experiments including proteomic/HCP analysis. This combination increases throughput by introducing high levels of automation while simultaneously reducing inter-operator variability.
“Expanding on many of the benefits of the Auto2D® device, our collaboration with TotalLab and the coupling of their SameSpots and SpotMap software in the Auto2D® downstream workflow further enhances the previously mentioned benefits. Requiring minimal parameter setting adjustments, TotalLab software reliably demonstrates high reproducibility of spot detection when coupled with the Auto2D® device.”
Keep reading
All SameSpots resources →- Application note2D gel quality control with SpotCheck: application note and 19-lab reproducibility studyHow SpotCheck in SameSpots measures 2D gel reproducibility against a gold standard: a within-lab application note and a 19-lab study in which 18 labs produced passing gels.
- GuideIsoelectric Focusing and IPG Strips Explained: The 2D Gel First DimensionHow isoelectric focusing separates proteins by pI, what IPG strips are and how to choose one, rehydration and voltage programs, and how to fix streaks and drift.
- ComparisonNormalization in 2D Gel and 2D-DIGE Analysis: Methods ComparedWhy 2D gel spot volumes must be normalized, how total volume, internal standard and log-based methods work, and what peer-reviewed comparisons say about them.
SameSpots2D gel and 2D-DIGE analysis with 100% spot matching and built-in statistics for differential expression.