Guide
Phoretix 1D User Manual
Please click the image below to access a full .pdf version of our user manual for our Phoretix 1D software:
If you’re currently using or trialing a copy of our Phoretix 1D software, please note you can always access a copy of this document from within the software either by pressing the F1 key or clicking on “help” within the options burger menu:
Or, if video tutorials are more your thing, you can access a full series of YouTube video tutorials on our official YouTube Channel here.

Keep reading
All Phoretix 1D resources →- GuideHow to capture quantitative western blot and gel imagesHow to capture western blot and gel images you can quantify: 16-bit grayscale, the linear range and the two kinds of saturation, choosing the exposure, resolution, file formats and a 12-point checklist.
- GuideTotal protein normalization for western blotsHow total protein normalization works, how stain-free, Ponceau S and fluorescent total protein stains compare, how to capture a valid total protein image, and how to normalize to total lane volume in Phoretix 1D.
- ComparisonImage Studio Lite replacement: your options in 2026What replaced Image Studio Lite, and how Image Studio, Empiria Studio and Phoretix 1D compare on imagers, lanes, validation, platforms, Part 11 and price.
Phoretix 1DAutomated lane and band detection, densitometry and normalization for 1D gels and western blots.


