SameSpots | How to assign protein isoelectric point and molecular weight to spots
Watch how by simply inputting your isoelectric point (pI) and molecular weight (MW) information into our SameSpots software it can then automatically calculate the pI and MW for all identified spots.
This is particularly useful in the identification of spots and can be combined with our MASCOT import to identify proteins on your gels or blots.
00:00 – Intro
00:35 – How to find pI and MW calibration tools
01:12 – IPG strip identification
01:25 – MW Ladder identification
01:42 – Navigating the Results Table
02:02 – How to add pI markers
02:20 – How to move pI markers once placed
02:22 – How to delete pI markers
02:29 – How pI and MW are calculated in SameSpots (piecewise linear)
03:50 – How to read pI of Spots from Results Table
04:07 – How to add MW markers
04:45 – How to read MW of Spots from Results Table
05:31 – Importing Protein List from MASCOT database
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