Image analysis resources library

Pattern Matching & Dendrogram Generation in CLIQS 1D Pro

Automate your 1D gel band matching and dendrogram generation with CLIQS 1D Pro. Build searchable gel databases and accelerate your electrophoresis pattern analysis.

Imaging 2D Gels: Essential Tips and Tricks

Discover expert insights on 2D gel electrophoresis imaging—a critical step in proteomic analysis. Learn best practices for high-resolution gel imaging, dynamic range optimization, and artifact prevention to ensure accurate, reproducible data. Brought to you by TotalLab, innovators in 2D proteomic analysis software.

Equipment & Reagents Required for Pulsed Field Gel Electrophoresis (PFGE)

Explore TotalLab's advanced PFGE equipment list designed for life scientists. Discover how our pulsed-field gel electrophoresis systems can deliver precise DNA separation for your research needs

HCP Coverage Analysis Using 2D Western Blot

Discover how 2D Western blotting enhances Host Cell Protein (HCP) coverage analysis in biopharmaceutical research. Learn the key steps, from protein separation and transfer to PVDF membranes to antibody staining and data analysis. See how TotalLab's SpotMap software improves HCP detection and quantification, ensuring regulatory compliance and accurate results.

TotalLab’s SpotMap: Automated 2D Western Blot Analysis Software for HCP Detection

How To Calculate Western Blot Normalization

What is the Precipitin Test?

Discover the science behind antigen-antibody precipitin reactions and how to analyze them using TotalLab's Phoretix 1D Software

How to Read Dot Blot Results

Learn to read dot blot results with easy steps and tips. Understand protein detection, antibody specificity, and optimize lab results

What are DNA Microarray Assays?

Discover what DNA microarray assays are, how they work, and their applications in genomics and personalized medicine.

What is Host Cell Protein?

Discover what host cell proteins (HCPs) are, why they matter in biologic drug development, and how they’re analyzed for safety. Learn about common HCP analysis methods, challenges, and strategies to manage risks, with insights on tools from Totallab

How to Read Western blot

Learn how to read Western blot results with easy, actionable steps. From sample preparation to data analysis, TotalLab's guide covers everything you need for accurate protein detection. Perfect for researchers and lab professionals!

SpotMap Examples and Evidence

We work closely with our customers from across the globe to ensure they get the best from SpotMap. Please find here a collection of examples and evidence of SpotMap being used by various Industrial and Academic customers and presented at conferences worldwide.

SDS-PAGE and Western Blot Image Capture Guide

Good image capture is critical to guarantee optimal performance of automated image analysis packages and generate reliable scientific data. This technical document provides a brief guide to the range of different image acquisition devices currently in use for gel applications, defines some the important technical factors required to generate digital images of a quality suitable for automated image analysis and recommendations for producing good 2D gel images.

TotalLab and Merck Collaborate to Automate 2D Gel Electrophoresis

By combining our industry leading SameSpots and SpotMap software with their Auto2D hardware, Merck have been able to rapidly reduce the time taken for complete 2D based experiments including proteomic/HCP analysis. This combination increases throughput by introducing high levels of automation whilst simultaneously reducing inter-operator variability.

The Essential Guide to 2D Electrophoresis

Learn how 2D electrophoresis can separate proteins by isoelectric point and molecular weight, aiding in analysis and research. Perfect for understanding complex protein mixtures and identifying biomarkers.

Bacterial Community Fingerprinting Protocol

Bacterial community fingerprinting refers to a number of cultivation-independent methods that are used to measure biodiversity in environmental samples or to profile microbial community structures, e.g. in response to experimental conditions. Read this guide to understand how you can use CLIQS 1D Pro in your bacterial fingerprinting studies.

2D-SDS PAGE Analysis Methods For Determining HCP Coverage Using Process Derived anti-HCP Polyclonal Antibodies

Here we present the characterization of a process specific anti-Pichia pastoris HCP antibody using 2 different analysis platforms: Biorad’s PDQuest and TotalLab’s SpotMap.

ALCOA Principles in Life Sciences and Pharmaceutical Production

Discover the essential ALCOA principles in life sciences and pharmaceutical production. Learn how Attributable, Legible, Contemporaneous, Original, and Accurate guidelines ensure data integrity, regulatory compliance, and patient safety. Understand the extended ALCOA+ principles and their critical role in the industry

Western Blot Troubleshooting Guide

The best methods for troubleshooting Western blots, including solutions for common issues like no signal, high background, non-specific bands, and uneven bands. Improve your Western blotting results with our detailed guide and visual aids

The 10 Golden Rules of GMP in Pharmaceutical Manufacturing

TotalLab – World Precision Instruments GMP Partnership

How to Implement 21 CFR Part 11 In Your Lab

Learn how TotalLab's software implements secure electronic records, robust audit trails, and user authentication protocols. Achieve compliance seamlessly with our expert guidance

Phoretix 1D | Background Removal Techniques For Western Blot

In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.

2D DIGE vs ELISA vs AAE for HCP Analysis

Explore the current landscape of anti-HCP antibody coverage with our article comparing 2D DIGE, ELISA, and AAE. Optimise your antibody selection process for robust anti-HCP analysis in biopharmaceutical research.

2D DIGE vs LC-MS/MS

Dive into the world of precision and versatility as we explore the distinct advantages offered by 2D DIGE and LC-MS/MS for comprehensive proteomic profiling.

2D DIGE vs 2D Gel Electrophoresis

In this short guide, we'll be explaining to you the technical process of performing 2D DIGE experiments and some of the advantages of using the technique over standard 2D electrophoresis.

CLIQS 1D Pro | How to create dendrograms for similarity

CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots. Here we demonstrate how to visualise the similarities between lanes in the form of dendrogams or similarity tables.

SameSpots | How to assign protein isoelectric point and molecular weight to spots

Learn how to add pI and MW info into SameSpots for automatic calculation of molecular weight for all identified spots.

CLIQs 1D Pro | How to Accurately Match Bands/Compare Lanes Across Different Gels and Blots

Watch how CLIQS 1D Pro can perform a simple comparison between lanes taken from different gels or blots, to identify any matches between them.

How to Calculate Host Cell Protein (HCP) Antibody Coverage % | SpotMap

This tutorial will show you how to analyse 2D gel electrophoresis and western blot images using TotalLab's SpotMap HCP image analysis software and calculate a percentage coverage score.

Phoretix 1D | How to Choose the Correct Background Removal Method

In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.

How to Automate Your Western Blot or Gel Electrophoresis Analysis

The automatic analysis features built into Phoretix 1D can save you huge amounts of time in a high-throughput environment and minimise inter-operator and inter-lab variability.

Phoretix 1D | How to Analyse Thin Layer Chromatography (TLC) Experiments

This tutorial will show you how to analyze thin layer chromatography slides/images using TotalLab's Phoretix 1D image analysis software.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Match Bands Across Your Database

In this video we show you how to perform a search of your blot or gel database to find matching bands across lanes, how to fine-tune your search parameters and how to express your matches statistically.

Phoretix 1D | How to Analyse a Western Blot

Following the launch of our Phoretix 1D image analysis software we've put together this tutorial video to show you how to analyse gel electrophoresis and blot images.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Save Sets of Lanes for Matching

In this video we'll be showing you how to create a set or group of existing lanes that you can repeatedly match across your entire 1D gel or Western blot database.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Create Lanes for Band Matching

In this video, we'll be showing you how to select an existing lane or create a new lane containing the bands you're interested in matching across your entire 1D gel or Western blot database.

CLIQS 1D Pro Image Database Software | Tutorial Series | How to Create a Database of Gel/Blot Images

In this video, we'll guide you through the first step in using our CLIQS 1D Pro software - the creation of a database containing lanes and bands extracted from 1D gels and blots analysed using our CLIQS 1D software.

SameSpots 2D Image Analysis Software | Tutorial Series | Image Quality Control

This video will show you the tools that are built directly into SameSpots to critically evaluate the quality of your 2D images.

SameSpots 2D Image Analysis Software | Tutorial Series | Multivariate Statistical Analysis of Spots

This video guides users through the statistical analysis of spots within 2D gels and Western blots using our SameSpots software.

SameSpots 2D Image Analysis Software | Tutorial Series | How to Align Images (Automatic & Manual)

This video outlines how SameSpots's next-generation image alignment algorithm is utilised to perfectly align 2D gel and Western blot images for statistical analysis in proteomic work.

Problem solving with SpotMap

Our guide covers some of the problems you can overcome when using our SpotMap software. It provides an introduction to key features, image QC, image analysis and details on the analysis element of the software.

How to Analyse 2D Gels

Discover the key steps of the SameSpots workflow and how it allows you to analyse 2D gels with any type of staining.

P-values, False Discovery Rate (FDR) and q-values

In this article we explore p-values, q-values and false discovery rates through example experiment results.

How to configure system security in Windows for GxP compliance

If you're operating in a 21 CFR part 11 compliance laboratory then setting up Windows security becomes a major part in setting up your system. Take a look at our tips.

Tips for setting up your electronic signature for the FDA

We have created these guidelines to support you in setting up your electronic signatures for FDA CFR part 11 compliance.

21 CFR Part 11 Compliance Guide

We have created this 21 CFR part 11 compliance guide to outline how our GxP Module software and the customer organisation share responsibilities for achieving compliance.

What Are Electronic Signatures for 21 CFR/GMP Compliance?

This guide provides on how you can use AuditSafe can ensure your electronic signatures are generated in compliance with 21 CFR Part 11/GMP regulations.

SpotMap | How to align Western blots to 2D gel images

Understanding when alignment is correctly implemented is easy in SpotMap as there are visual identifiers in the software that indicate when images are ready for analysis.

How to Correctly Align 2D Gels and Western Blots for HCP Analysis

Alignment can be changed and corrected at any point on the analysis however there are four ways to check you have Align your images correctly.

21 CFR part 11/GMP compliance checklist

We have created this 21 CFR part 11 compliance checklist to ensure you are have the right checks in place to comply with the regulations.