Discover expert insights on 2D gel electrophoresis imaging—a critical step in proteomic analysis. Learn best practices for high-resolution gel imaging, dynamic range optimization, and artifact prevention to ensure accurate, reproducible data. Brought to you by TotalLab, innovators in 2D proteomic analysis software.
Read document >Explore TotalLab's advanced PFGE equipment list designed for life scientists. Discover how our pulsed-field gel electrophoresis systems can deliver precise DNA separation for your research needs
Read document >Discover how 2D Western blotting enhances Host Cell Protein (HCP) coverage analysis in biopharmaceutical research. Learn the key steps, from protein separation and transfer to PVDF membranes to antibody staining and data analysis. See how TotalLab's SpotMap software improves HCP detection and quantification, ensuring regulatory compliance and accurate results.
Read document >Discover the science behind antigen-antibody precipitin reactions and how to analyze them using TotalLab's Phoretix 1D Software
Read document >Learn to read dot blot results with easy steps and tips. Understand protein detection, antibody specificity, and optimize lab results
Read document >Discover what DNA microarray assays are, how they work, and their applications in genomics and personalized medicine.
Read document >Discover what host cell proteins (HCPs) are, why they matter in biologic drug development, and how they’re analyzed for safety. Learn about common HCP analysis methods, challenges, and strategies to manage risks, with insights on tools from Totallab
Read document >Learn how to read Western blot results with easy, actionable steps. From sample preparation to data analysis, TotalLab's guide covers everything you need for accurate protein detection. Perfect for researchers and lab professionals!
Read document >We work closely with our customers from across the globe to ensure they get the best from SpotMap. Please find here a collection of examples and evidence of SpotMap being used by various Industrial and Academic customers and presented at conferences worldwide.
Good image capture is critical to guarantee optimal performance of automated image analysis packages and generate reliable scientific data. This technical document provides a brief guide to the range of different image acquisition devices currently in use for gel applications, defines some the important technical factors required to generate digital images of a quality suitable for automated image analysis and recommendations for producing good 2D gel images.
Read document >By combining our industry leading SameSpots and SpotMap software with their Auto2D hardware, Merck have been able to rapidly reduce the time taken for complete 2D based experiments including proteomic/HCP analysis. This combination increases throughput by introducing high levels of automation whilst simultaneously reducing inter-operator variability.
Read document >Learn how 2D electrophoresis can separate proteins by isoelectric point and molecular weight, aiding in analysis and research. Perfect for understanding complex protein mixtures and identifying biomarkers.
Read document >Bacterial community fingerprinting refers to a number of cultivation-independent methods that are used to measure biodiversity in environmental samples or to profile microbial community structures, e.g. in response to experimental conditions. Read this guide to understand how you can use CLIQS 1D Pro in your bacterial fingerprinting studies.
Read document >Here we present the characterization of a process specific anti-Pichia pastoris HCP antibody using 2 different analysis platforms: Biorad’s PDQuest and TotalLab’s SpotMap.
Read document >Discover the essential ALCOA principles in life sciences and pharmaceutical production. Learn how Attributable, Legible, Contemporaneous, Original, and Accurate guidelines ensure data integrity, regulatory compliance, and patient safety. Understand the extended ALCOA+ principles and their critical role in the industry
Read document >The best methods for troubleshooting Western blots, including solutions for common issues like no signal, high background, non-specific bands, and uneven bands. Improve your Western blotting results with our detailed guide and visual aids
Read document >Learn how TotalLab's software implements secure electronic records, robust audit trails, and user authentication protocols. Achieve compliance seamlessly with our expert guidance
Read document >In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.
Read document >Explore the current landscape of anti-HCP antibody coverage with our article comparing 2D DIGE, ELISA, and AAE. Optimise your antibody selection process for robust anti-HCP analysis in biopharmaceutical research.
Read document >Dive into the world of precision and versatility as we explore the distinct advantages offered by 2D DIGE and LC-MS/MS for comprehensive proteomic profiling.
Read document >In this short guide, we'll be explaining to you the technical process of performing 2D DIGE experiments and some of the advantages of using the technique over standard 2D electrophoresis.
Read document >CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots. Here we demonstrate how to visualise the similarities between lanes in the form of dendrogams or similarity tables.
Watch video >Learn how to add pI and MW info into SameSpots for automatic calculation of molecular weight for all identified spots.
Watch video >Watch how CLIQS 1D Pro can perform a simple comparison between lanes taken from different gels or blots, to identify any matches between them.
Read document >This tutorial will show you how to analyse 2D gel electrophoresis and western blot images using TotalLab's SpotMap HCP image analysis software and calculate a percentage coverage score.
Watch video >In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.
Watch video >The automatic analysis features built into Phoretix 1D can save you huge amounts of time in a high-throughput environment and minimise inter-operator and inter-lab variability.
Watch video >This tutorial will show you how to analyze thin layer chromatography slides/images using TotalLab's Phoretix 1D image analysis software.
Watch video >In this video we show you how to perform a search of your blot or gel database to find matching bands across lanes, how to fine-tune your search parameters and how to express your matches statistically.
Watch video >Following the launch of our Phoretix 1D image analysis software we've put together this tutorial video to show you how to analyse gel electrophoresis and blot images.
Watch video >In this video we'll be showing you how to create a set or group of existing lanes that you can repeatedly match across your entire 1D gel or Western blot database.
Watch video >In this video, we'll be showing you how to select an existing lane or create a new lane containing the bands you're interested in matching across your entire 1D gel or Western blot database.
Watch video >In this video, we'll guide you through the first step in using our CLIQS 1D Pro software - the creation of a database containing lanes and bands extracted from 1D gels and blots analysed using our CLIQS 1D software.
Watch video >This video will show you the tools that are built directly into SameSpots to critically evaluate the quality of your 2D images.
Watch video >This video guides users through the statistical analysis of spots within 2D gels and Western blots using our SameSpots software.
Watch video >This video outlines how SameSpots's next-generation image alignment algorithm is utilised to perfectly align 2D gel and Western blot images for statistical analysis in proteomic work.
Watch video >Our guide covers some of the problems you can overcome when using our SpotMap software. It provides an introduction to key features, image QC, image analysis and details on the analysis element of the software.
Read document >Discover the key steps of the SameSpots workflow and how it allows you to analyse 2D gels with any type of staining.
Read document >In this article we explore p-values, q-values and false discovery rates through example experiment results.
Read document >If you're operating in a 21 CFR part 11 compliance laboratory then setting up Windows security becomes a major part in setting up your system. Take a look at our tips.
Read document >We have created these guidelines to support you in setting up your electronic signatures for FDA CFR part 11 compliance.
Read document >We have created this 21 CFR part 11 compliance guide to outline how our GxP Module software and the customer organisation share responsibilities for achieving compliance.
Read document >This guide provides on how you can use AuditSafe can ensure your electronic signatures are generated in compliance with 21 CFR Part 11/GMP regulations.
Read document >Understanding when alignment is correctly implemented is easy in SpotMap as there are visual identifiers in the software that indicate when images are ready for analysis.
Watch video >Alignment can be changed and corrected at any point on the analysis however there are four ways to check you have Align your images correctly.
Watch video >We have created this 21 CFR part 11 compliance checklist to ensure you are have the right checks in place to comply with the regulations.
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