Generative AI is rapidly changing the lab, but can it write your next compliant product? Discover the right - and wrong - times to use AI code generation in life science software, and why human expertise remains absolutely vital for GMP compliance and data security.
Read document >Transitioning to mass spectrometry for impurity testing often means drowning in complex data. Learn how SpotMap MS simplifies LC-MS HCP analysis with "hands-free" automated workflows, an AI-categorized threat database, and reporting tools purpose-built for biopharma QC.
Read document >Normalization is the most critical step for achieving accurate, publication-ready quantitative data. In this comprehensive guide, we take a deep dive into the theory and practice of 1D gel and Western blot normalization. Discover the differences between total protein and housekeeping gene methods, and learn how Phoretix 1D software eliminates user bias to automate your entire analysis workflow.
Read document >CLIQS 1D Pro case studies and evidence showing how labs use 1D gel band matching for microbial community fingerprinting and rapid wheat varietal identification.
Read document >See how SameSpots 2D gel analysis software helps leading proteomics labs cut analysis time, improve gel alignment and generate more robust biomarker data.
Read document >Learn how to apply Hi3 label-free quantitation in SpotMap MS for accurate, reproducible HCP quantification from LC-MS data.
Read document >Discover how SpotMap MS automates high-risk HCP identification from LC-MS data using intelligent AI-database matching.
Read document >Relying on Excel macros for 21 CFR Part 11 and GMP compliance exposes your lab to malware, tampering and audit findings. Macro-based plugins are hard to validate, difficult to control and a known cyber attack vector. Discover how TotalLab’s AuditSafe adds secure audit trails, electronic signatures and granular permissions to Excel - delivering compliant, validated workflows without relying on risky VBA code.
Read document >Discover how AI-powered, digital CQV platforms help pharma QC teams replace paper-based validation with compliant, data-driven workflows. Learn how to reduce review time, prevent deviations earlier, and align with 21 CFR Part 11 and EU Annex 11.
Read document >TotalLab is now a Scientist.com supplier, giving pharma and biotech teams an easier, compliant route to custom life science and OEM software development.
Read document >Explore detailed case studies, posters, and testimonials highlighting the performance and reliability of SpotMap MS software. Designed for automated LC-MS based host cell protein (HCP) analysis, SpotMap MS is trusted by pharmaceutical researchers for accuracy, reproducibility, and ease of use. Use these resources to confidently evaluate how SpotMap MS can support your lab’s QC and research workflows.
Read document >The AI regulatory landscape is evolving rapidly, creating complex compliance challenges for organisations. This guide helps TotalLab clients navigate the EU AI Act, UK's pro-innovation approach and sector-specific requirements. Learn how TotalLab can help with your risk assessment, documentation, governance and building compliant AI systems from the ground up.
Read document >SpotMap MS empowers pharmaceutical analysis of host cell proteins via LC-MS QC with advanced ion intensity maps for rapid detection of sample issues, ensuring reliable and compliant data.
Read document >Phoretix 1D outperforms Gel Analyzer with advanced automation, accurate band detection, and regulatory compliance. Discover why labs are upgrading to Phoretix 1D for faster, more reliable gel image analysis in 2025. Experience smarter workflows and precise quantification with this industry-leading gel analysis software.
Read document >Immunoaffinity chromatography enhances detection and purification of host cell proteins, delivering reliable results for biopharmaceutical quality control.
Read document >Are you currently using or trialing a copy of TotalLab's Phoretix 1D SDS-PAGE analysis software? Find a full user manual/guide here.
Read document >Host Cell Protein (HCP) impurity analysis is essential for safe biologics manufacturing. Discover how TotalLab’s software tools help to streamline HCP quantification when using gold-standard techniques like ELISA, 2D-PAGE, and LC-MS to support regulatory compliance and therapeutic safety.
Read document >Understanding your HCP antibody coverage is essential to validating your ELISA for host cell protein detection. This article explains how coverage impacts assay reliability, regulatory compliance, and effective antibody selection in biologics and biosimilar development.
Read document >Learn how TotalLab software supports accurate, contamination-free 2D protein spot excision and downstream mass spectrometry analysis. From 2D-DIGE to Western blot workflows, our tools streamline detection, quantification, and protein ID.
Read document >Following the discontinuation of Bio-Rad's PDQuest software, Bio-Rad now recommends TotalLab’s SameSpots and SpotMap 2D as trusted replacements for 2D gel image analysis and HCP antibody validation. Learn how these solutions offer modern workflows for differential expression and coverage scoring.
Read document >Automate your 1D gel band matching and dendrogram generation with CLIQS 1D Pro. Build searchable gel databases and accelerate your electrophoresis pattern analysis.
Read document >Discover expert insights on 2D gel electrophoresis imaging—a critical step in proteomic analysis. Learn best practices for high-resolution gel imaging, dynamic range optimization, and artifact prevention to ensure accurate, reproducible data. Brought to you by TotalLab, innovators in 2D proteomic analysis software.
Read document >Explore TotalLab's advanced PFGE equipment list designed for life scientists. Discover how our pulsed-field gel electrophoresis systems can deliver precise DNA separation for your research needs
Read document >Discover how 2D Western blotting enhances Host Cell Protein (HCP) coverage analysis in biopharmaceutical research. Learn the key steps, from protein separation and transfer to PVDF membranes to antibody staining and data analysis. See how TotalLab's SpotMap software improves HCP detection and quantification, ensuring regulatory compliance and accurate results.
Read document >Discover SpotMap: automated 2D Western blot analysis software for host cell protein detection. Enhance accuracy and streamline gel image workflows.
Read document >Learn how to calculate Western blot normalization, compare HKP and total protein methods, and boost accuracy in protein quantitation with TotalLab's Phoretix 1D software.
Read document >Discover the science behind antigen-antibody precipitin reactions and how to analyze them using TotalLab's Phoretix 1D Software
Read document >Learn to read dot blot results with easy steps and tips. Understand protein detection, antibody specificity, and optimize lab results
Read document >Discover what DNA microarray assays are, how they work, and their applications in genomics and personalized medicine.
Read document >Discover what host cell proteins (HCPs) are, why they matter in biologic drug development, and how they’re analyzed for safety. Learn about common HCP analysis methods, challenges, and strategies to manage risks, with insights on tools from Totallab
Read document >Learn how to read Western blot results with easy, actionable steps. From sample preparation to data analysis, TotalLab's guide covers everything you need for accurate protein detection. Perfect for researchers and lab professionals!
Read document >Explore detailed case studies, posters, and testimonials highlighting the performance and reliability of SpotMap 2D software. Designed for automated 2D-PAGE host cell protein coverage analysis, SpotMap 2D is trusted by pharmaceutical researchers for accuracy, reproducibility, and ease of use. Use these resources to confidently evaluate how SpotMap 2D can support your lab’s QC and research workflows.
Read document >Good image capture is critical to guarantee optimal performance of automated image analysis packages and generate reliable scientific data. This technical document provides a brief guide to the range of different image acquisition devices currently in use for gel applications, defines some the important technical factors required to generate digital images of a quality suitable for automated image analysis and recommendations for producing good 2D gel images.
Read document >By combining our industry leading SameSpots and SpotMap software with their Auto2D hardware, Merck have been able to rapidly reduce the time taken for complete 2D based experiments including proteomic/HCP analysis. This combination increases throughput by introducing high levels of automation whilst simultaneously reducing inter-operator variability.
Read document >Learn how 2D electrophoresis can separate proteins by isoelectric point and molecular weight, aiding in analysis and research. Perfect for understanding complex protein mixtures and identifying biomarkers.
Read document >Bacterial community fingerprinting refers to a number of cultivation-independent methods that are used to measure biodiversity in environmental samples or to profile microbial community structures, e.g. in response to experimental conditions. Read this guide to understand how you can use CLIQS 1D Pro in your bacterial fingerprinting studies.
Read document >Here we present the characterization of a process specific anti-Pichia pastoris HCP antibody using 2 different analysis platforms: Biorad’s PDQuest and TotalLab’s SpotMap.
Read document >Discover the essential ALCOA principles in life sciences and pharmaceutical production. Learn how Attributable, Legible, Contemporaneous, Original, and Accurate guidelines ensure data integrity, regulatory compliance, and patient safety. Understand the extended ALCOA+ principles and their critical role in the industry
Read document >The best methods for troubleshooting Western blots, including solutions for common issues like no signal, high background, non-specific bands, and uneven bands. Improve your Western blotting results with our detailed guide and visual aids
Read document >Learn how TotalLab's software implements secure electronic records, robust audit trails, and user authentication protocols. Achieve compliance seamlessly with our expert guidance
Read document >In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.
Read document >Explore the current landscape of anti-HCP antibody coverage with our article comparing 2D DIGE, ELISA, and AAE. Optimise your antibody selection process for robust anti-HCP analysis in biopharmaceutical research.
Read document >Dive into the world of precision and versatility as we explore the distinct advantages offered by 2D DIGE and LC-MS/MS for comprehensive proteomic profiling.
Read document >In this short guide, we'll be explaining to you the technical process of performing 2D DIGE experiments and some of the advantages of using the technique over standard 2D electrophoresis.
Read document >CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots. Here we demonstrate how to visualise the similarities between lanes in the form of dendrogams or similarity tables.
Watch video >Learn how to add pI and MW info into SameSpots for automatic calculation of molecular weight for all identified spots.
Watch video >Watch how CLIQS 1D Pro can perform a simple comparison between lanes taken from different gels or blots, to identify any matches between them.
Read document >This tutorial will show you how to analyse 2D gel electrophoresis and western blot images using TotalLab's SpotMap HCP image analysis software and calculate a percentage coverage score.
Watch video >In this demo, we explain the difference between our background removal techniques and how to choose the correct one based on your data.
Watch video >The automatic analysis features built into Phoretix 1D can save you huge amounts of time in a high-throughput environment and minimise inter-operator and inter-lab variability.
Watch video >This tutorial will show you how to analyze thin layer chromatography slides/images using TotalLab's Phoretix 1D image analysis software.
Watch video >In this video we show you how to perform a search of your blot or gel database to find matching bands across lanes, how to fine-tune your search parameters and how to express your matches statistically.
Watch video >Following the launch of our Phoretix 1D image analysis software we've put together this tutorial video to show you how to analyse gel electrophoresis and blot images.
Watch video >In this video we'll be showing you how to create a set or group of existing lanes that you can repeatedly match across your entire 1D gel or Western blot database.
Watch video >In this video, we'll be showing you how to select an existing lane or create a new lane containing the bands you're interested in matching across your entire 1D gel or Western blot database.
Watch video >In this video, we'll guide you through the first step in using our CLIQS 1D Pro software - the creation of a database containing lanes and bands extracted from 1D gels and blots analysed using our CLIQS 1D software.
Watch video >This video will show you the tools that are built directly into SameSpots to critically evaluate the quality of your 2D images.
Watch video >This video guides users through the statistical analysis of spots within 2D gels and Western blots using our SameSpots software.
Watch video >This video outlines how SameSpots's next-generation image alignment algorithm is utilised to perfectly align 2D gel and Western blot images for statistical analysis in proteomic work.
Watch video >Our guide covers some of the problems you can overcome when using our SpotMap software. It provides an introduction to key features, image QC, image analysis and details on the analysis element of the software.
Read document >Discover the key steps of the SameSpots workflow and how it allows you to analyse 2D gels with any type of staining.
Read document >In this article we explore p-values, q-values and false discovery rates through example experiment results.
Read document >If you're operating in a 21 CFR part 11 compliance laboratory then setting up Windows security becomes a major part in setting up your system. Take a look at our tips.
Read document >We have created these guidelines to support you in setting up your electronic signatures for FDA CFR part 11 compliance.
Read document >Compliance with FDA 21 CFR Part 11 is critical for life science laboratories relying on electronic records and signatures. In this comprehensive technical guide, we break down how our AuditSafe software helps users achieve the regulatory requirements of Subpart B, EU Annex 11, GMP and ALCOA data integrity principles. Download the guide to discover how TotalLab's AuditSafe software seamlessly enforces compliance and secures your digital workflows.
Read document >This guide provides on how you can use AuditSafe can ensure your electronic signatures are generated in compliance with 21 CFR Part 11/GMP regulations.
Read document >Understanding when alignment is correctly implemented is easy in SpotMap as there are visual identifiers in the software that indicate when images are ready for analysis.
Watch video >Alignment can be changed and corrected at any point on the analysis however there are four ways to check you have Align your images correctly.
Watch video >We have created this 21 CFR part 11 compliance checklist to ensure you are have the right checks in place to comply with the regulations.
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